HDAC6在ISO诱导大鼠心肌纤维化中的作用研究

摘要

Objective The experiment was to investigate the expression of HDAC 6 in the rat model of myocardial fibrosis induced by ISO.Methods SD rats were randomly divided into 2 groups,each group of 20 which to set up the normal group and model group.The model group was treated with ISO daily by 5mg/kg for 7 d,the another group was given the same amount of saline.Take its blood and myocardial specimens.And tested its Heart quality index.ELISA was used to detected the content of type I and type III collagen.USed HE staining and Masson staining to observe the degree of myocardial fibrosis.The expression of HDAC6 andα-SMA were tested by Western blot.The mRNA expression ofα-SMA and HDAC6 were examined by qRT-PCR.Results Compared with model group,heart mass index was increased ( <0.05);HE staining and Masson staining showed collagen fiber hyperplasia in model group;Collagen type I and III in model group have increased relatively ( <0.05);Western blot showed that protein expression in model group was higher than normal group ( <0.05),α-SMA expression is obviously higher than normal control group ( <0.01);QRT-PCR detection showed that mRNA expression in model group was higher than normal group ( <0.05).Conclusion HDAC6 in plays an important role in development and incidence of myocardial fibrosis.%目的：本实验探讨HDAC 6在ISO诱导的大鼠心肌纤维化动物模型中表达机制。方法将SD大鼠随机分为两组，每组各20只。设正常组和模型组。其中模型组的大鼠予以诱导剂ISO 1次/d[5mg/(kg·d)]皮下注射，另一组给予等剂量的生理盐水，持续7d。取其血液和心肌标本，测定其心脏质量指数；应用酶联免疫吸附法检测I型和III型胶原的含量；采用HE和Masson染色法观察大鼠心肌纤维化程度；蛋白质免疫印迹法测定HDAC 6和α-SMA蛋白的表达情况；采用qRT-PCR技术检测HDAC 6的mRNA的表达情况。结果模型组和正常对照组相比，心脏质量指数明显增加(<0.05)；HE染色和Masson染色显示模型组胶原纤维增生。I、III型胶原较对照组有相对明显增加(<0.05)；Western blot法示模型组蛋白表达显著大于正常组(<0.05)，而且α-SMA的表达显著大于正常组(<0.01)；qRT-PCR检测模型组中mRNA表达明显大于正常组(<0.05)。结论 HDAC6在心肌纤维化的发生发展中发挥着重要作用。