微波辅助萃取-液相色谱-串联质谱法检测猪骨汤中氯霉素残留

摘要

A method for determination of chlorainphenicol residues in pig bone soup by microwave - assisted extraction technique combined with liquid chromatography -tandem mass spectrometry was established in this paper. The sample was extracted by microwave extraction equipment, dried with evaporation equipment, then purified with C18 solid phase extraction cartridge, and finally carried out by multiple re- actions monitoring mode and calculated by internal standard method. The pretreatment conditions and clean - up and all the parameters of instrument were optimized. The recovery was 91.5% -101%. The detection limit was 0. 1 g/kg and the correlation coefficient of linear regression equations was 0. 9996.%建立了微波辅助萃取一液相色谱一串联质谱法（LC／MS／MS）检测猪骨汤中氯霉素残留的方法。样品经微波萃取，浓缩至近干，采用自制C18固相萃取小枉净化后上机检测，在多反应监测模式下检测，内标法定量，并优化了提取、净化及仪器各项参数：方法回收率为91．5％～101％，线性方程的相关系数为0．9996，定量限为0．1g／kg。