Electrochemiluminescence(ECL) based on Ru(bpy)32+ immobilized modified electrode has attracted extensive attention in molecular biology, chemistry, medicine and other fields due to its versatility, inherent height detection sensitivity and simplified optical setup. The use of new materials to overcome the poor stability of the original method and the disadvantage of poor reproducibility has broadened the bipyridine ruthenium immobilized ECL range of applications. The new materials, methods and their application for immobilized Ru(bpy)32+ over the past decades have been systematically reviewed.%固定联吡啶钌电致化学发光体系因其灵敏度高、可以连续测试、装置简单等优点被广泛应用于分子生物学、化学、药学等领域.各种新材料的运用克服了原有方法稳定性差、重现性差等缺点,拓宽了固定化联吡啶钌电致化学发光的应用范围.介绍了近几年来一些用于固定联吡啶钌新的材料、固定方法及其相关的应用.
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