综述了固定化联吡啶钌[Ru(bpy)2+3+]电化学发光免疫传感技术的发展状况,介绍了近年来在电化学发光免疫传感领域出现的新型固相载体材料和固定方法,及其与其他分析技术联用方面的发展,并对其发展趋势进行了展望.指出电化学发光免疫分析技术在生物分子检测、药物分析及临床诊断中显示出强大的生命力.Ru(bpy)2+3的电化学发光已引起广泛的关注.固定化Ru(bpy)2+3电化学发光免疫分析体系具有线性范围宽、灵敏度高、装置简单、可控性强等优点,被广泛应用于分子生物学、化学、药学等领域.%A review is given of the research progress of immobilized tris(2, 2'-bipyridyl) ruthenium (II) [Ru(bpy)3+] for electrochemiluminescen.ee immunoassay (ECLIA) . Recently developed novel solid-phase supports and immobilization methods in the field of electrochemilumi-' nescence immunosensing as well as their coupling with other analytical techniques are introduced, and the future prospect of ECLIA is proposed. It is pointed out that eleetrochemilumi-nescence analysis technique is a useful tool widely used in biological molecular monitoring, pharmaceutical analysis and clinic diagnosis, and the electrochemiluminescence of Ru(bpy)3+ has attracted a great deal of interest. Particularly, immobilized Ru(bpy)3+ has been widely applied in the fields of molecular biology, chemistry, and pharmacy, due to its advantages such as wide linear range, high sensitivity, simplicity and good controllability in association with immunosensing.
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