羊栖菜不同分子质量褐藻多酚抗氧化活性研究

摘要

The antioxidative activities of phlorotannins with different molecular weight extracted from Sargassum fusiforme were investigated. The prussian blue method was used to detect their total oxidant capacity, and DPPH, hydroxyl free radical kit and superoxide anion kit were used to measure their scavenging ability, respectively. The results showed that S. fusiforme mainly contains high molecular phlorotannins. Components from ethyl acetate layer exhibited stronger antioxidant capacities than those from water layer. The component with molecular weight more than 30000 from ethyl acetate layer exhibited stronger hydroxyl free radicals and superoxide anion scavenging ca-pabilitythan gallic acid, Vc and VE.%　　将羊栖菜(Sargassum fusiforme)经浸提、萃取和超滤等步骤获得不同分子质量的褐藻多酚。用普鲁士蓝法检测其总抗氧化能力；采用 DPPH、羟自由基试剂盒、超氧阴离子试剂盒评价其对 DPPH、羟自由基和超氧阴离子的清除能力。结果显示，羊栖菜褐藻多酚主要以高分子质量的形式存在；乙酸乙酯相组分各项抗氧化指标均好于水相，且乙酸乙酯相分子质量大于30000组分对羟自由基与超氧阴离子的清除能力比没食子酸、Vc和VE强。