三角褐指藻紫外线诱变及高产EPA藻株选育

摘要

利用紫外诱变法对三角褐指藻(Phaeodactylum tricornutum)进行诱变育种.实验得到三角褐指藻的最佳紫外辐射剂量为18 W的紫外灯距离藻液35 cm照射15 min.通过单细胞分离技术获得1株突变株UP1,与出发藻株相比,突变株UP1的EPA产量提高10.2％.研究了诱变株的最适生长及产EPA的条件,结果表明UP1在NaNO375 mg/L,pH 7.5,昼夜温度17～15℃,接种量为10％时培养7天,具有最大的生长速率和EPA产量.探讨了诱变株的遗传稳定性,结果表明诱变株可稳定遗传.%This study investigated the mutagenesis of Phaeodactylum tricornutum induced by UV irradiation and its capacity of EPA accumulation influenced by various cultivation factors. The appropriate ultraviolet irradiation dose that can be applied toP. tricornutum was found to be below 35 cm for 15 min. A special clone UP1 was isolated from more than 200 mutant clones. Compared with the wild species, the EPA content of mutant UP1 was increased by about 10.2％. The effects of NaNO3 concentration, pH of the medium, and temperature on EPA production were investigated, which showed that the optimal cultivation conditions were NaNO375 mg/L, pH 7.5, natural dayight temperature about 17–15℃, and 10％ of inoculation density for 7 days of cultivation. The mutant species demon-strated inherited stable.