室外池塘自然养殖条件下呋喃西林代谢物在中华绒螯蟹体内残留和消除规律

摘要

Elimination rules of nitrofuranzone metabolite Semicarbazide (SEM) in Eriocheir sinensis were investigated.The concentration of SEM in tissues (muscle,hepatopancreas,gill) were analyzed by liquid chromatography tandem mass spectrometric (HPLC-ESI/MS/MS).Eriocheir sinensis were drug bathed in nitrofuranzone liquid (20 mg · L-1 and 80 mg · L-1),respcetively.After 1 h,the samples were washed and collected at 1 h,2 h,4 h,8 h,12 h,24 h,2 d,4 d,8 d,12 d,16 d,20 d,30 d,40 d,50 d,60 d,80 d,100 d,120 d and 150 d.The results showed that the peak time in the tissues was 2 h after drug bath.The peak concentration of SEM in gill,muscle and hepatopancreas were (152 ± 21.7),(234.0 ± 12.0) and (3 160 ±169)μg· kg-1 in 20 mg· L-1 drug bathed group,while (327± 31.2),(372 ± 27.2) and (4 623 ± 247) μg · kg-1 in 80 mg · L-1 drug bathed group.SEM in the tissues of Eriocheir sinensis presented different degradations,the average degradation of SEM were 0.315 (in muscle),0.487 (in hepatopancreas) and 4.39 (in gill) μg · (kg · h)-1 in 20 mg · L-1 drug bathed group.The average degradation of SEM were 0.454 (in muscle),0.516 (in hepatopancreas) and 6.43 (in gill) μg · (kg · h)-1 in 80 mg · L-1 drug bathed group.960 h after drug bath,SEM residual of two groups were lower than 1.0 μg.%采用高效液相色谱串联质谱(HPLC-ESI/MS/MS)法分析呋喃西林主要代谢物氨基脲(Semicarbazide,SEM)在中华绒螯蟹(Eriocheir sinensis,以下简称河蟹)体内的残留及消除规律.设2个试验组和1个对照组,2个试验组以20 mg·L-1和80 mg·L-1的呋喃西林溶液浸泡河蟹(扣蟹)60 min后,转移至饲养池塘中,并在给药后1h、2h、4h、8h、12 h、24 h、2d、4d、8d、12 d、16 d、20 d、30 d、40 d、50 d、60 d、80 d、100 d、120 d、150 d进行各组织取样分析.结果显示:在停药2h时,2个浓度试验组河蟹的肌肉、肝胰腺和鳃中SEM均达到高峰,其中,20 mg·L-1试验组测定值分别为(152±21.7)、(234.0±12.0)、(3 160±169) μg·kg-1,80 mg·L-1试验组测定值分别为(327±31.2)、(372±27.2)、(4 623±247) μg·kg-1.停药2h后,2个浓度试验组河蟹各组织中SEM均呈现不同的消除速率,其中20 mg·L-1试验组的消除速率分别为0.315μg·(kg·h)-1(肌肉)、0.487 μg· (kg·h)-1(肝胰腺)和4.39 μg·(kg·h)-1(鳃);80 mg·L-1试验组的消除速率分别为0.454 μg· (kg· h)-1(肌肉)、0.516 μg· (kg·h)-1(肝胰腺)和6.43 μg· (kg· h)-1(鳃).停药960 h后,2个浓度试验组各组织中SEM残留均低于判定限(1.0 μg·kg-1).