呋喃西林代谢物在杂交鳢(斑鳢♀×乌鳢♂)体内的残留消除规律研究

摘要

在模拟养殖条件下,对杂交鳢(斑鳢Channa maculata♀×乌鳢Channa argus♂)体内呋喃西林代谢物氨基脲(Semicarbazide,SEM)的残留及消除规律进行了研究.在(30.5±0.5)℃水温条件下,用质量浓度为2 mg/L呋喃西林药浴杂交鳢苗种2d.药浴期间于0.5、1、2、4、8、12、24、48 h取样,停止药浴后于0.5、1、2、4、8、12、24、48、96、192、288、384、480、720、960、1 200、1 440、1 920、2 400、2 880 h取样,采用液-质联用(HPLC-MS)方法测定杂交鳢肌肉中SEM的含量.结果显示,药浴期间0.5～48 h,肌肉中SEM质量分数从(32.58±2.82) μg/kg逐渐升高至(175.92±18.49) μg/kg;药浴结束后的SEM质量分数经测定于1h达到最高,为(108.00 ±5.86) μg/kg;药浴结束后2～ 48h,苗种体内SEM质量分数维持在(53.60 ±4.90) ～ (69.21 ±5.71)μg/kg,48 h后显著下降,至60d后已低于检测限0.25 μg/kg.鱼体肌肉中SEM的平均消除速率为0.089 μg/(kg.h).结果显示,在该试验条件下,鱼体内SEM可在60 d后代谢消除.%In this paper, the residues and depuration of semicarbazide ( SEM) in hybrid snakeheads (Channa maculata (♀×) × Channa argus (♂ ) ) were tested. Hybrid snakeheads were drug-bathed 2 days with 2 mg/L nitrofurazone at temperatures of (30. 5 ± 0. 5) ℃. During drug-bath, the samples were collected at 0.5,1,2,4,8,12,24,48 h,and at 0.5,1,2,4,8,12,24,48,96,192,288,384,480,720, 960,1 200,1 440,1 920,2 400,2 880 h after drug-bath, and the SEM residues in muscle were measured by high performance liquid chromatography tandem mass spectrometry ( HPLC-MS). The results showed that the SEM residue from 0. 5 to 48 h in hybrid snakehead muscle increased gradually from (32. 58 ± 2. 82) to( 175. 92 ± 18. 49) μg/kg during drug-bath. SEM residue was found to be the highest, (108. 00 ± 5.86) ±g/kg at 1 h after drug-bath. The residues ranged between (53. 60 ± 4. 90) and (69. 21 ±5.71) μ/kg from 2 to 48 h after drug-bath but significantly decreased after 48 h, which was below the detection limit of 0. 25 μg/kg at 1 440 h. The average degradation of SEM in muscle was 0. 089 μg/(kg o h). The results showed that the nitrofurazone of muscle in this experimental condition was degraded after 60 days.