To explore a novel method to establish a thin endometrium by injecting ethanol into the uterine cavity of rat. Twenty-five female SD rats were divided into three groups: control group (physiological saline were injected into cavities and kept for 5 min), 5 minutes group (95% ethanol was injected and kept for 5 min) and 10 minutes group (95% ethanol was injected and kept for 10 min). The endometria which were thin-ner than that of the control group would be taken as a new group-model group, the others were control group. Observe endometrial morphology by hematoxylin-eosin staining (HE) and analyze the growth of the epithelial and stroma cells, VEGF and Era expression by immunohistochemieal staining method. Fourteen u-terine endometria thinned in 5 minutes group and the endometrial layers even all uterine layers of 16 uteri were necrosed in 10 minutes group. All endometria were characterized by poor regrowth in model group. Compared with control group, the cytokeratin and the vimentin area per unit endometrium area was less (P< 0.05); VEGF expressed lower in ndometrium (P<0.05); the expression of Era was the same (P>0.05) between two groups. A rat model of thin endometrium was successfully established when 95% ethyl alcohol was kept in uterine cavities for 5 min, and the success rate was 70%.%为探索宫腔注入无水乙醇建立薄型子宫内膜模型的可行性,将25只大鼠分3组:对照组(宫腔注入生理盐水),5分钟组(宫腔注入无水乙醇并贮留5 min),10分钟组(宫腔注入无水乙醇并贮留10 min),经测量子宫内膜变薄者为造模组,5分钟组有14个子宫内膜变薄,10分钟组无一例纳入造模组.免疫组织化学检测角蛋白、波形蛋白、血管内皮生长因子(vascular endothelial growth factor,VEGF)、雌激素受体α(estrogen related receptor alpha,ERα)的表达.结果发现5分钟组造模组与对照组单位内膜面积中角蛋白面积、单位间质面积中波形蛋白面积和子宫内膜中VEGF平均光密度差异有统计学意义(P＜0.05),造模组与对照组子宫内膜ERα组织学积分无差异(P＞0.05).表明宫腔注入无水乙醇并贮留5 min可以成功建立薄型子宫内膜模型,成功率为70％.
展开▼
