Rat Models and Identification of Candidate Early Serum Biomarkers of Battlefield Traumatic Brain Injury; Final rept. 1 Jul 2005-30 Apr 2007

摘要

This study is part of a larger multi-year study that has two main specific aims: 1. the characterization of a 'closed head' FPI(lateral fluid percussion), versus a penetrating ballistic rat model of traumatic brain injury (TBI) and 2. the identification of specific plasma proteomic biomarkers of TBI. Funding has covered year 1 of this study, and the following related objectives: 1) Application of TBI, neurobehavioral testing and collection of biofluids and tissues; 2) Optimization of proteomic protocols for the identification of differentially expressed proteins in the plasma of brain-traumatized rats; 3) Preliminary identification of proteins differentially expressed in plasma from rat models of TBI. All the objectives set for this part of the larger study have been met. One hundred thirty nine Sprague-Dawley rats have been used to achieve Objective 1. Results show a significant neurobehavioral effect of both kinds of TBI, more drastic for the PBI model. Optimization of proteomic technology (Objective 2) has allowed the analysis of replicate samples collected at the different time points following injury. We have thus met Objective 3 by identifying several potential time-dependent plasma protein biomarkers of TBI, including many proteins known to be normally expressed in the central nervous system. Future evaluations will need to include the verification of some of the most statistically and biologically significant plasma proteome changes observed in this study and evaluation of their dependence on trauma intensity.