重要转录因子FoxO3a在斑马鱼性腺中的表达分析

摘要

FoxO蛋白作为Forkhead Box家族的重要一员,在动物的生长发育、细胞分化、代谢、免疫和凋亡等方面起重要作用,研究发现FoxO蛋白是哺乳动物卵泡发育的重要调节因子.通过检测雌雄斑马鱼性腺中foxo3a基因的表达情况,初步研究FoxO在鱼类生殖发育中的作用.PCR和蛋白免疫杂交结果都显示foxo3a基因在斑马鱼雌性性腺中的表达量要明显高于雄性.这一结果提示,foxo3a基因在斑马鱼的卵巢发育中可能发挥重要作用.%As a crucial member in the Forkhead Box family, FoxO plays a major role in the animal growth, cell differentiation, metabolism, immunization and apoptosis. FoxO was found to be an essential regulatory factor in the follicular development in mammalian. The expression of the foxo3a gene was detected by reverse transcriptase technology and Western blotting for studying the function of FoxO in the fish reproductive development. It's revealed that the expression level of foxoia in the ovary is higher than that in the spermary in zebrafish. The results indicated that the transcription factor Fox03α may play an important role in ovarian development in fish.