人细胞角蛋白Keratin14的生物信息学分析

摘要

人细胞角蛋白Keratin 14是Ⅰ型中间丝蛋白家族的亚群,参与形成上皮细胞的细胞骨架.利用生物信息学分析Keratin 14基因的启动子区、CpG岛及其所编码的蛋白质的理化性质、结构特点和功能特征.结果表明,Keratin 14基因存在两个启动子区,启动子区不存在CpG岛;Keratin 14全长472个氨基酸,其等电点为5.09,是一个无跨膜结构的亲水性蛋白质;角蛋白Keratin 14二级结构具7个α螺旋和5个β折叠,预测三级结构的拉曼图分析显示其结构稳定;与Keratin 14相互作用的蛋白质主要是角蛋白,而且Keratin 14还参与细胞的生长和分裂,有促进细胞分化的作用;此外,Keratin 14的氨基酸序列在哺乳动物间的同源性较高.上述对角蛋白Keratin 14的表达、结构和功能的预测可以为研究其在生命过程中的作用提供重要的信息.%Keratin 14 is a member of the type I intermediate filament protein family,and involves in the formation of epithelial cell cytoskeleton.Here,bioinformatic analysis was performed to analyze the promoter and CpG islands of Keratin 14 gene,as well as the physical and chemical properties,structural and functional characteristics of Keratin 14 protein.The results showed that there are two promoters and no CpG island in the gene.In addition,Keratin 14 protein is composed of 472 amino acids,with isoelectric point of 5.09,and is a hydrophilic protein without a signal peptide and transmembrane domain.Furthermore,seven α-helices and fiveβ-sheets were predicted in the secondary structure,and the structure was proven to be stable by Ramachandran plot.From the function perspective,Keratin 14 not only interacts with Keratin but also plays an essential role in regulating cell growth and division,prompting cell differentiation.The homologies of the amino acid sequence to those of other mammals are very high.The analysis results provide important messages for further studies on function of Keratin 14 in human life activities.