苹果 MD-ACO1启动子的克隆

摘要

以苹果（Malus pumila Mill．）品种“皇家嘎拉”（Rolay Gala）叶片为材料，采用 CTAB 的方法提取总 DNA。根据 GenBank 中已发表的苹果 ACO1启动子的 DNA 序列，设计引物。采用 PCR 法，克隆该启动子的 DNA 片断。得到了长度分别为953bp 和975bp 的 DNA 片段。DNA 测序分析软件分析结果显示：该序列与已登录的片断的核苷酸序列一致性分别为98％、97％，认为该基因为苹果 ACO1的启动子序列。%Using the technic of CTAB,the whole DNA in apple(Malus pumila Mill.)cultivar ‘Rolay Gala’ was cloned.According to the apple ACO1 promoters gene that has published in the GenBank,constructing primer.Using PCR method Cloned the promoter fragments.A ACO1 promoter fragment which contains 953 bp and 975 bp was cloned.The sequence consistency rates of nucleotide was 98%,97%.We concluded that this gene fragment was MD-ACO1 promoter.