单独或联合应用辛酸沉淀、饱和硫酸铵沉淀、阴离子交换等方法对羊抗人IgG进行纯化，对纯化前后抗体的纯度和免疫学活性进行比较，并与辣根过氧化物酶连接，作为二抗用于抗-HCV的ELISA检测。结果表明，不同方法纯化的抗体其纯度和免疫学活性具有一定程度的差别，其中经辛酸+饱和硫酸铵沉淀纯化的抗体为最佳，凝胶扫描纯度为98.05%，比活性近1 800，为纯化前的6.8倍。用辣根过氧化物酶标记后，作为酶标二抗检测HCV阴性和阳性标准血清各40份，阴性符合率为97.5%，阳性符合率为95%，可用于抗-HCV的ELISA检测。%Sheep-anti-human IgG was purified by caprylic acid precipitation, ammonium sulfate precipitation and anion exchange chromatography, separately or jointly. The purity and immunoactivity of the purified antibodies were measured by SDS-PAGE and ELISA, respectively. The purified IgG were labelled with HRP and used as secondary antibody in the ELISA detection of anti-HCV. The results indicated that the purity and immunoactivity varied according to different purification methods. IgG purified by caprylic acid precipitation plus ammonium sulfate precipitation is the best for further use with 98.05% purity and 1800 ratio activity. HRP-IgG was used to test 40 HCV negative and 40 HCV positive standard serum. The negative conformity rate is 97.5% and positive conformity rate is 95%. It can be used for ELISA test of anti-HCV.
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