藏獒MC1R基因多态性与毛色表型的关联研究

摘要

目的:通过检测藏獒黑素皮质激素受体1(MC1R)基因的单链构象多态性(SSCP)在不同毛色群体中的分布,探讨MC1R基因多态性与毛色表型的相关性.方法:采用DNA测序技术,选择不同毛色藏獒的DNA为样本,根据GenBank发布的荷斯坦牛MC1R基因序列设计一对引物,采用PCR-SSCP技术分析MC1R基因在藏獒中的SSCP.结果:MC1R基因在藏獒中具有PCR-SSCP多态性,分别检测到3种基因型(AA、AB和BB)；对MC1R基因多态性片段DNA克隆测序后发现,MC1R基因在编码区第313位存在单碱基突变(G→A),该突变导致第105位氨基酸发生由丙氨酸向苏氨酸的改变(T105A).结论:MC1R基因的多态性与毛色性状不存在显著的相关性.%Objective: To evaluate the relationship between the melanocortin receptor 1(MC1R) gene and coal color by comparing single-strand conformation polymorphism (SSCP) of Tibetan mastiff with different coat colors. Methods: DNA extracted from specimens of Tibetan mastiff with different coat colors were analyzed and se-quenced. A pair of primers was designed by sequence of MCIR gene of Holstein published in GenBank. Results: The MCIR gene of SSCP in the Tibetan mastiff was detected through PCR-SSCP. The polymorphism was found with two alleles A and B and three genotypes AA, AB and BB. The polymorphic fragments were sequenced. According to the results of sequencing, the mutation (G313A) in CDS of MCIR gene was found and it altered amino acid at the position 105 from alanine to threonine. Conclusion: There is no notable correlation between SNP of MCIR gene and coat color.