目的建立能实际用于临床的测定血浆非蛋 白结合铁的方法。方法选取正常和窒息新生儿 各10例，分别用校正和传统博来霉素法测定 其脐血浆非蛋白结合铁含量，并比较两者的标准曲线、回收率和t检验结果 。结果传统博 来霉素法测定血浆非蛋白结合铁用于临床血样时，回收率只有32%左右，基体效应明显。结 果不可靠用基体血浆克服基体效应后，发现正常和窒息新生儿脐血浆非蛋白结合铁含量差别 增大，t检验差异具显著性。且回收试验提示回收率达101%左右。结论用克服基体效应的 博来霉素法测得的血浆非蛋白结合铁其数值客观反映临床实际情况，且方法稳定可靠。%Objective To establish a practical method of detecting cord plasma nonprotein bound iron(NPBI). M ethods Of 20 cord plasma samples,10 of mormal, 10 of asphyxial newborns, respectively, their NPBI were investigated by traditional and corrected blo emycin assay. Their standard curves, recovery rates and t-test statistical results were compared. Results We found the cord plasma NPBI were lower detected by tra ditional bleomycin assay in normal and asphyxial newborns, their recovery rate was only about 32%. There is an obviousely matrix effect. Though the NPBI of normal and asphyxial ne wborns had still significant difference by t-test,the NPBI values were so l ow th at it's easily disturbed and difficult to practice on clinical use. The detecte d results were improved by matrix effec-free bleomycin assay. Their rec overy rat es were reached about 101% and the NPBI values had statistical significant d iffe rence between normal and asphyxial newborn. Conclusion The matri x effec-free bleo mycin assay is a stable, real and better method to estimate the concentration of plasma NPBI practically on clinical use.
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