一株产H2S变异产肠毒素性大肠埃希菌O15:K?的鉴定与初步研究

摘要

Objective To investigate preliminarily the biological characteristics of H2S-producing Escherichia coli.Methods The unknown H2S-producing Escherichia coli isolated from clinical stool specimen of a child with diarrhoea was identified for morphology ,culture characteristics, biochemical reaction and serological identification. Drug resistance tests were determined by disk diffusion method. Results The bacterium was identified as H2S-producing enterotoxigenic Escherichia coli (ETEC)O15∶K? and was sensitive to most antibacterial drugs. Conclusions The H2 S-producing ETEC O15 ∶K? is the variant lapactic Escherichia coli which needs further investigation. The clinic and the laboratories should pay much attention to this variation.%目的 对新发现的1株产H2S大肠埃希菌进行细菌学鉴定,并对其生物学特性进行初步研究.方法 对临床1例腹泻病患儿粪便标本中分离的产H2S大肠埃希菌采用形态学、培养特性、生化反应、血清学等方法 进行鉴定,并用纸片扩散法进行药物敏感性试验.结果 经全面鉴定,该菌为产H2S的产肠毒素性大肠埃希菌(ETEC)O15:K?,耐药性不强,对大多数抗菌药物敏感.结论 产H2S的ETEC O15:K? 是变异的致泻性大肠埃希菌,应引起临床和实验室的高度重视.致泻性大肠埃希菌产H2S变异的现象值得进一步深入研究.