儿童肠道感染中致泻性大肠埃希菌的检测

摘要

目的了解儿童肠道感染中致泻性大肠埃希菌的感染情况。方法对95份腹泻患儿粪标本中分离出的大肠埃希菌，用荧光聚合酶链反应（PCR）的方法检测毒力基因，鉴定致泻性大肠埃希菌。结果检测出15株阳性菌株，阳性率为15．8％，其中肠聚集性大肠埃希菌（EAEC）6株、肠致病性大肠埃希菌（EPEC）4株、弥散聚集性大肠埃希菌（DAEC）4株和肠产毒性大肠埃希菌（ETEC）1株。结论致泻性大肠埃希菌是引起儿童腹泻的主要致病菌，临床实验室应加强对致泻性大肠埃希菌的检测。%Objective To analyze the infection status of diarrheagenic Escherichia coli (E .coli) from children with intestinal infection .Methods Virulence gene was detected and diarrheagenic E .coli was identified in 95 strains of E .coli isolated from children with intestinal infection .Results In 95 samples ,the positive rate was 15 .8% (15/95) ,including 6 strains of enteroaggarative E .coli ,4 strains of enteropathogenic E .coli ,4 strains of diffusely adherent E .coli and 1 strain of enterotoxigenic E .coli .Conclusion Diarrheagenic E .coli could be the main pathogens causing children diarrhea ,for which the detection test should be emphasized in clinical laboratory .