慢性丙型肝炎患者病毒基因分型及HCV RNA相关性研究

摘要

目的：探讨江苏地区慢性丙型肝炎患者病毒基因分型特点及其与患者年龄、性别和丙型肝炎病毒（HCV）RNA水平的关系。方法收集抗 HCV 阳性血清1032份，采用荧光定量 PCR（Q‐PCR）法检测患者血清HCV RNA水平，HCV基因分型芯片进行基因分型检测，应用SPSS19．0软件对数据进行统计分析。结果检测出HCV RNA阳性样本704份，其中 HCV 单基因型674份，1b、2a、3b、3a、1a 和6型分别占75．71％、7．95％、7．95％、1．99％、0．99％和1．14％；混合基因型30份，1b／2a、1b／3b、1b／3a和2a／3b型分别占2．27％、1．56％、0．28％和0．14％。不同基因型均以男性和青壮年居多，且病毒载量差异有统计学意义：1b和3a相近，都高于2a和1b／2a病毒载量。结论江苏地区 HCV基因型以1b型为主，其次是2a、3b型。慢性丙型肝炎患者以中青年男性居多，而且不同 HCV基因型间病毒载量有差异。%Objective To investigate the distribution of hepatitis C virus (HCV ) genotypes in Jiangsu Prov‐ince ,and the relationship between HCV genotypes and HCV viral loads .Methods Serum specimens ,collected from 1 032 anti‐HCV positive samples ,were detected for HCV RNA by using fluorescent quantitative polymerase chain reaction (PCR) and for HCV genotypes by using gene chips .The distribution and characterization of HCV genotypes as well as the relationship between genotypes and HCV RNA content were analyzed by SPSS 19 .0 software . Results A total of 704 HCV RNA positive serum samples were collected ,among which 674 cases were found to be single genotype ,including 1b ,2a ,3b ,3a ,1a and 6 ,accounting for 75 .71% ,7 .95% ,7 .95% ,1 .99% ,0 .99% and 1 .14% ,respectively ,and 30 cases of mixed genotypes ,including 1b/2a ,1b/3b ,1b/3a and 2a/3b ,accounting for 2 .27% ,1 .56% ,0 .28% and 0 .14% .The difference of viral loads among different HCV genotypes was significant . Conclusion Genotype 1b might be the most prevalent genotype in Jiangsu ,followed by 2a and 3b .HCV genotype 1b and 3a could be with similar viral loads ,which is higher than genotype 2a and 1b/2a significantly .