IL-33和ST2在慢加急性乙型肝炎肝衰竭和慢性乙型肝炎患者中水平差异及应用研究

摘要

Objective To discuss and study on the differences in the levels of IL‐33 and ST2 between the patients with chronic liver failure with acute hepatitis B (ACHBLF) and chronic hepatitis B and application value .Methods Forty‐two cases of patients with ACHBLF treated in our hospital were selected as the study group A ,and another 45 cases of patients with chronic hepatitis B were selected as the study group B ,then 45 cases of healthy volunteers carried out normal physical examination during the same pe‐riod were as the healthy control group .The serum IL‐33 and ST2 levels were detected by the ELIAS ,and the results were com‐pared .The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ,albumin (ALB) levels in the three groups were detected by Lai′s method and ELISA method and bromocresol green method respectively ,and the clinical correlations between the levels of serum IL‐33 and ST2 and the levels of ALT ,AST and ALB in the study group A and study group B were analyzed respec‐tively .Results The levels of serum IL‐33 and ST2 ,ALT and AST in the study group A were significantly higher than those in the study group B and control group (P<0 .05) ,which the study group B were significantly higher than those in the healthy control group (P<0 .05) .The level of ALB in the study group A was significantly lower than that in the study group B and the healthy control group (P<0 .05) ,which in the study group B was significantly lower than that in the healthy control group (P<0 .05) .The levels of serum IL‐33 and ST2 in the study group A were positively related to the levels of ALT and AST ,which were negatively related to the level of ALB .The levels of serum IL‐33 and ST2 in the study group B were positively related to the levels of ALT and AST ,which had no obvious correlations to the level of serum ALB .Conclusion The serum levels of IL‐33 and ST2 in patients with ACHBLF were significantly higher than that of patients with chronic hepatitis B ,suggesting that it may be associated with increased inflammatory response and liver injury .%目的：探讨白细胞介素‐33（IL‐33）和ST‐2蛋白（ST2）在慢加急性乙型肝炎肝衰竭（ACHBLF）和慢性乙型肝炎患者中水平差异及应用价值。方法选取该院42例ACHBLF患者作为研究组A组，45例慢性乙型肝炎患者作为研究B组，另选取45例同期于该院行健康体检的健康志愿者作为健康对照组。采用酶联免疫吸附试验对血清IL‐33和S T 2水平进行检测，并对比检测结果。分别采用赖氏法、酶联免疫吸附试验、溴甲酚绿法对3组研究对象丙氨酸氨基转移酶（ALT ）、天门冬氨酸氨基转移酶（AST ）、清蛋白（ALB）水平进行检测，并分析在研究A组和研究B组中血清IL‐33和ST2分别与ALT、AST 和ALB的临床关系。结果研究A组血清IL‐33和ST2、ALT和AST水平较研究B组和健康对照组显著升高，差异均有统计学意义（P＜0．05），且研究B组IL‐33和ST2、ALT和AST水平也较健康对照组显著升高，差异有统计学意义（P＜0．05）；研究A组血清ALB水平较研究B组和健康对照组显著降低，差异有统计学意义（P＜0．05），且研究B组ALB水平显著低于健康对照组，差异有统计学意义（P＜0．05）；研究A组血清IL‐33和ST2与ALT、AST水平呈显著正相关，与血清ALB呈显著负相关；研究B组血清IL‐33和ST2与ALT、AST水平呈显著正相关，与血清ALB无明显相关性。结论 ACHBLF患者血清IL‐33和ST2水平较慢性乙型肝炎患者显著升高，推测可能与加重炎性反应和肝损伤有关。