山东地区448例丙型肝炎患者HCV基因分型状况分析

摘要

目的 研究丙型肝炎病毒(HCV)基因分型,了解山东地区丙型肝炎的基因分型情况.方法 收集448例丙型肝炎患者的血清标本,采用焦磷酸测序法对HCV感染标本进行分型.结果 448例丙型肝炎患者共检出7种基因亚型,分别为1b型288例(64.28%)、2a型137例(30.57%)、1a型13例(2.90%)、3b型4例(0.90%)、3a型2例(0.45%)、6a型2例(0.45%)、1a1b型2例(0.45%);对HCV基因型与性别、年龄的关系进行分析显示无明显相关性;2013-2016年各基因型患病率分析结果显示无明显的变化趋势.结论 山东地区HCV感染基因型以1b型为主,其次为2a型;HCV基因型分布与性别、年龄差异无统计学意义(P>0.05),且近4年山东地区各基因型年患病率无明显变化趋势.%Objective To study the genotype of hepatitis C virus(HCV) and understand the genotype distribution of hepatitis C in Shandong area.Methods Serum samples from 448 patients with hepatitis C were collected and classified into HCV infected samples by pyrosequencing.Results Totally 448 cases of patients with hepatitis C were detected in 7 genotypes,respectively,288 cases of type 1b(64.28%),137 cases of type 2a(30.57%),13 cases of type 1a(2.90%),4 cases of type 3b(0.90%),2 cases of type 3a(0.45%),2 cases of type 6a(0.45%),2 cases of type 1a1b(0.45%).The analysis of HCV genotype and sex and age showed no significant correlation.The analysis of genotype prevalence in 2013-2016 years showed no obvious change trend.Conclusion Hepatitis C virus infection in Shandong area is dominated by type 1b,followed by type a2;there was no significant difference in age and gender,in the past 4 years,there is no obvious change trend of genotype annual prevalence in Shandong area.