Th17免疫细胞受细菌生物膜影响后水平变化的研究

摘要

Objective To investigate the significance of Th17 cell and IL-17 level change affect by bacterial biofilm.Methods Two hundreds and sixty-one specimens of alveolar lavage fluid from January to December 2015 were selected to conduct bacterial culture and identification.The Th17 cell expression rate and IL-17 cytokine of peripheral blood and alveolar lavage fluid were detected in healthy population,patients with and without biofilm bacterial infection.Then the analysis and comparison were performed.Results The Th17 cell expression rate and IL-17 cytokine level had statistical difference btween the patients with biofilm bacterial infection and the patients with non-biofilm bacterialinfection(P<0.05);however there are no statistical differences in the Th17 cell expression rate and IL-17 cytokine level of peripheral blood between these two groups and healthy population(P>0.05).Conclusion Bacterial biofilm could stimulate the increase of Th17 cell expressing level and IL-17 cytokine level in infected part,but could not cause the systemic immunoreaction,which has a clinical guiding significance.%目的 探讨辅助性T细胞17(Th17)和白细胞介素 (IL)-17受细菌生物膜影响引起水平变化的意义.方法 选取2015年1-12月肺泡灌洗液标本261例进行细菌培养与鉴定,分别检测健康人群、生物膜细菌感染人群及非生物膜细菌感染人群外周血和肺泡灌洗液的Th17细胞表达率和IL-17细胞因子,并进行分析比较.结果 生物膜感染人群肺泡灌洗液中Th17细胞表达率及IL-17细胞因子水平与非生物膜感染人群比较差异有统计学意义(P<0.05);而这2组外周血与健康人群外周血的Th17细胞表达率及IL-17细胞因子水平之间比较差异无统计学意义(P>0.05).结论 细菌生物膜可以刺激机体感染局部Th17细胞表达水平和IL-17细胞因子水平升高,而并未引起全身免疫反应,具有临床指导意义.