体外诱导树鼩骨髓间充质干细胞向神经元样细胞分化的实验研究

摘要

目的 探讨树鼩骨髓间充质干细胞(BM-MSCs)向神经元样细胞分化的可行性.方法 通过采用不同诱导物浓度的神经元样细胞诱导液对体外培养的树鼩BM-MSCs向神经元样细胞进行诱导分化,用PCR法检测细胞神经元烯醇化酶(NES)和α-突触核蛋白(α-SYN)表达情况,并用NES抗体进行细胞免疫荧光染色鉴定.结果 使用浓度为10 ng/mL表皮生长因子(EGF)和20 ng/mL碱性成纤维细胞生长因子(bFGF)诱导液在体外诱导培养树鼩BM-MSCs至12d时可以得到良好诱导结果.结论 优化的双细胞因子诱导培养液可以成功将树鼩BM-MSCs诱导分化为神经元样细胞,分化的细胞符合神经元样细胞的一般特征.%Objective To observe the feasibility of the differentiation of tree shrew (Tupaia belangeri) bone marrow mesenchymal stem cells (BM-MSCs) to neuron-like cells.Method The tree-shrew BM-MSCs were differentiated to neuron-like cells by IMEM differentiation culture mediums (with different inducer and concentration).Identify the secretion concentration of neuron-specific enolase (NES) and α-synuclein (α-SYN) by PCR.NES antibody was also used to identify cell immunofluorescence.Result The result of the tree-shrew BM-MSCs differentiation to neuron-like cells with IMEM cell culture differentiation mediums (10 μg/mL EGF and 20 μg/mL bFGF) were good on 12d in-vitro.Conclusion Optimized cnlture medium with double grow factors and differentiation method were more appropriated for tree shrew BM-MSCs differentiation to neuron-like cells,and the cells differentiated by this manner were consistent to neuron-like cells characteristics.