自发性糖尿病长爪沙鼠环氧化酶(COX)-2在三种组织中的表达

摘要

Objective To analyze Cyclooxygenase 2(COX-2) expression level in different tissues of diabetic gerbils and to explore the relationship of diabetes occurrence and development in Mongolian gerbils.Methods Different tissues including skeletal muscle,liver and kidney were collected from 6 diabetic gerbils and 6 control animals respectively.COX-2 mRNA and protein expression level in different tissues were separately detected by Real-time PCR and Western blotting.Results The Real-time PCR showed that compared with control animal,the mRNA expression of COX-2 in skeletal muscle had no obvious difference whereas it exhibited uptrend in liver and kidney.As the results of Western blotting,the difference of COX-2 in skeletal muscle was almost undetectable in diabetes group.The relative expression level in diabetic group exhibited uptrend compared with control in liver and kidney.And there was statistical significance in the kidney.Conclusions COX-2 was high expressed in liver and more remarkably in kidney of diabetic gerbils.It may illustrate that the effect of COX-2 mainly occurred in liver and kidney of diabetic gerbils.%目的 研究环氧化酶(COX)-2基因在糖尿病长爪沙鼠3种组织中的表达水平,进一步探索其与长爪沙鼠糖尿病发生和发展的关系.方法 选取糖尿病长爪沙鼠和正常沙鼠各6只,分别采集动物的骨骼肌、肝脏和肾脏组织,用实时PCR和Western blotting检测COX-2基因在各组织中mRNA和蛋白表达水平.结果 实时PCR的结果表明,COX-2基因的mRNA水平在糖尿病组动物的骨骼肌组织中表达与对照组无明显差异,而肝脏和肾脏组织中有表达升高趋势.Western blotting结果显示,糖尿病组COX-2的蛋白水平在肝脏和肾脏组织中存在表达升高趋势,且肾脏具有统计学意义.结论 COX-2在糖尿病长爪沙鼠肝脏和肾脏组织中表达升高,在肾脏组织尤其明显,说明COX-2对长爪沙鼠糖尿病的影响可能发生在肝脏和肾脏组织中.