新生儿缺氧缺血性脑病患儿外周血CD4+CD25+调节性T细胞的变化及其意义

摘要

Objective To explore the changes of CD4 + CD25 + regulatory T cells in peripheral blood of neonatal patients with hypoxic-ischemic encephalopathy (HIE),and its significance in the process of HIE.Methods The levels of CD4 + CD25 + Tcells and changes of the foxp3 expression in 92 patients with HIE and 31 health children were detected by flow cytometry.Results The levels of CD4 + CD25 + T cells in the peripheral blood of moderate and severe HIE neonates were significantly lower than that of controls (P ＜ 0.05).The foxp3 expression in CD4 + CD25 + T cells in peripheral blood of moderate and severe HIE neonatal were significantly lower than that the controls(P ＜ 0.05).Conclusion The CD4 + CD25 + foxp 3 + T cell in moderate and severe HIE children are significantly reduced,and these regulatory T cells may be involved in the pathological process of HIE.%目的 探讨CD4+CD25+调节性T细胞在新生儿缺氧缺血性脑病(HIE)患儿外周血的改变,及其在HIE疾病进程中的意义.方法 用流式细胞术分析HIE组患儿92例和对照组31名外周血CD4+ CD25+T细胞的变化,及其细胞内foxp3的表达变化.结果 轻度、中度和重度HIE组患儿外周血CD4+ CD25+T细胞比例分别为(9.26±1.18)％、(6.51±1.35)％和(5.36±1.54)％,对照组为(8.42±2.02)％.轻度HIE组患儿外周血CD4+ CD25+T细胞较对照组有增高趋势,但差异无统计学意义(P＞0.05)；中度和重度HIE组患儿CD4+ CD25+T细胞明显低于对照组,差异有统计学意义(P＜0.05).轻度、中度和重度HIE组患儿外周血CD4+CD25+foxp3+T细胞分别为(1.36±1.14)％、(1.21±0.94)％和(1.09±0.54)％,对照组为(1.30±1.17)％.轻度HIE组患儿CD4+ CD25+ foxp3+T细胞较对照组有增高趋势,但差异无统计学意义(P＞0.05).中度和重度HIE组患儿CD4+CD25+foxp3+T细胞明显低于对照组,差异有统计学意义(P＜0.05).结论 中度和重度HIE组患儿CD4+CD25+foxp3+T细胞明显减少,这些调节性T细胞可能参与HIE的病理进程.