Objective To study the clinical value of using immunofluorescence method in digestive malignant tumor to dectect self-directed antinuclear antibody.Methods The study cases were from our hospital in January 2015-January 2016,85 cases of patients with digestive system malignant tumor,85 cases of benign digestive system cancer patients as a disease control group,85 cases of healthy physical examination as a control,using indirect immunofluorescence method for patients' antinuclear antibody test.We then compared the three groups' of antinuclear antibody test results,and cell fluorescence localization results.Results ① Metastasispositive rate and no transfer group positive rate was no significant different,P ＞ 0.05;Malignant tumor group is significantly higher than benign tumor group and control group,x2 =16.940,37.531,P =0.000,0.000;Benign tumor group were significantly higher than control group,x2 =5.328,P =0.021).②Between malignant tumor and benign tumor group,benign tumor group and the control group,the cytoplasmic antibody fluorescence localization rate had no significant difference,(P ＞ 0.05).Compared to he control group,malignant tumor,benign tumor group antinuclear antibody fluorescence localization rate was significantly higher,x2 =5.221,20.299,P =0.022,0.000;Malignant tumor resistance cytoplasmic antibody fluorescence localization rate is significantly higher,x2 =13.523,P =0.000).Compared to benign tumor group,malignant tumor group antinuclear antibody fluorescence localization rate was significantly higher,so did the anti-cytoplasmic antibody,x2 =5.842,8.947,P =0.016,0.003.③Between malignant tumor metastasis group and no transfer group,both the antinuclear antibody and anti-cytoplasmic antibody,fluorescence localization rate has no obvious difference,(P＞0.05).Conclusion Using the immunofluorescence method for the patients with digestive malignant tumor,the antinuclear antibody testing has important clinical values.It can effectively evaluate the disease and treatment outcomes,providing a basis for clinical immunity treatment.%目的 探讨免疫荧光法在结直肠肿瘤自身抗核抗体检测中的临床价值.方法 选取我院2015年1月至2016年1月85例结直肠癌患者为恶性肿瘤组,85例结直肠良性肿瘤患者为良性肿瘤组,85例健康体检者为对照组.采用间接免疫荧光法对受试者的抗核抗体进行检测,对比3组抗核抗体检测及在细胞内荧光定位的结果.结果 ①恶性肿瘤组中转移组抗核抗体阳性率和无转移组阳性率比较无明显差异(P＞0.05);恶性肿瘤组抗核抗体阳性率明显高于良性肿瘤组和对照组x2=16.940、37.531,P=0.000、0.000;良性肿瘤组抗核抗体阳性率高于对照组,x2=5.328,P=0.021.②恶性肿瘤组和良性肿瘤组、对照组抗胞质抗体荧光阳性率无明显差异(P＞0.05).和对照组比较,恶性肿瘤组、良性肿瘤组抗核抗体荧光阳性率明显升高x2=5.221、20.299,P=0.022、0.000;恶性肿瘤抗胞质抗体荧光阳性率明显升高,x2=13.523,P =0.000.和良性肿瘤组比较,恶性肿瘤组抗核抗体和抗胞质抗体荧光阳性率明显升高,x2=5.842、8.947,P=0.016、0.003.③恶性肿瘤转移组和无转移组抗核抗体和抗胞质抗体荧光阳性率无明显的差异,不具有统计学意义(P＞0.05).结论 采用免疫荧光法对消化系统恶性肿瘤患者的自身抗核抗体进行检测具有重要的意义,能够有效地判断肿瘤的病情和治疗效果,为临床免疫性治疗提供一定的依据.
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