乳腺浸润性导管癌组织中磷脂酶A2蛋白的表达

摘要

Aim: To explore the relationship between the expressions of Spla2- II a and Cpla2 protein and the occurrence, development, invasion and metastasis of breast invasive ductal carcinoma( BIDC). Methods:Immunohistochemical SP method was used to detect the expressions of Spla2- II a and Cpla2 proteins in 57 cases of BIDC.15 cases of breast fi-broadenoma and 15 cases of normal breast tissue. Results:The positive expression rates of Spla2-II a(75. 4% ) and Cp-LA2(68.4% ) proteins in B1DC tissue were higher than those in breast fibroadenoma(20. 0% , 26.7% ) and normal breast tissue ( 13.3%, 13. 3% ) , there was significant difference among the three groups^2 =27. 585 and 18. 989 ,P <0. 001) . The expressions of Spla2-II a (x2 =6.428,16. 336,and 6. 007,P <0. 05) and Cpla2(x2 = 10. 072,7. 140,and 3. 959, P <0.05) protein were all associated with the clinical grade, histological grade and lymphatic metastasis in BIDC tissue. There was positive relationship between the protein expressions of Spla2- II a and Cpla2 ( rp =0. 373 , P = 0. 002 ). Conclusion :Spla2-II a and Cpla2 play important roles in the infiltration,metastasis and carcinogenesis of BIDC,and could become molecular indexes in the early diagnosis and prognostic judgement.%目的:探讨分泌型磷脂酶A2(sPLA2)-Ⅱ a及胞浆型磷脂酶A2(cPLA2)蛋白的表达与乳腺浸润性导管癌发生发展及浸润、转移的关系.方法:应用免疫组织化学SP法检测57例乳腺浸润性导管癌组织、15例乳腺纤维腺瘤组织及15例正常乳腺组织中sPLA2-Ha及cPLA2蛋白的表达.结果:乳腺浸润性导管癌组织中sPLA2-lIa(75.4%)及cPLA2蛋白(68.4%)的阳性表达率与乳腺纤维腺瘤组织(20.0%,26.7%)及正常乳腺组织(13.3%,13.3%)比较,差异均有统计学意义(χ2=27.585及18.989,P均<0.001).乳腺浸润性导管癌组织中sPLA2-Ⅱa及cPLA2蛋白的阳性表达率与临床分期、组织学分级及有无淋巴结转移均有关(sPLA2-Ⅱa:χ2=6.428、16.336和6.007,P均<0.05;cPLA2:x2=10.072、7.140和3.959,P均<0.05),且二者表达有关(rp=0.373,P=0.002).结论:sPLA2-Ⅱa及cPLA2在乳腺浸润性导管癌的浸润、转移及癌变过程中起重要作用,有望成为早期诊断和判断预后的分子指标.