替米沙坦对大鼠心肌肥厚组织中RASSF1A表达的影响

摘要

Aim: To investigate the effect of telmisartan on expression of RASSF 1A in hypertrophy myocardial tissue of rats induced by isoproterenol. Methods: Thirty rats were randomly divided into three groups and 10 rats in each group : control group,model group(3 mg· kg-1· d-1 isoproterenol for 10 d) and intervention group(3 mg· kg -1 · d-1 isoproterenol and 8. 33 mg· kg -1 · d-1 telmisartan for 10 d). RASSF1A mRNA and protein in left ventricular tissues were analyzed by RT-PCR and immunohistochemistry ,respectively. Results ;The expressions of RASSF 1A mRNA and protein in the 3 groups were statistically different ( F = 38. 968 ,284. 445 ,respectively ,P < 0. 001). The above indexes in the model group and in -tervention group were lower than those of the control group (P < 0. 05) , but those of the intervention group were higher than the model group (P < 0. 05 ). Conclusion:Telmisartan can inhibit myocardial hypertrophy induced by isoproterenol through regulating the expression of RASSF1A%目的:观察替米沙坦对心肌肥厚大鼠左室心肌组织中RASSF1A表达的影响.方法:SD大鼠30只,随机分为对照组、模型组和干预组.模型组和干预组给予异丙肾上腺素3 mg/(kg·d)皮下注射,干预组在建模同时灌胃替米沙坦8.33 mg/(kg·d).10 d后,进行血流动力学和心肌肥厚指数测定;取大鼠左心室心肌组织,分别采用RT-PCR和免疫组化法检测RASSF1A mRNA 和蛋白的表达,同时观察病理学变化.结果:血流动力学、心室肥厚指数及病理观察结果提示,模型组大鼠发生心肌肥厚,干预组上述变化较模型组减轻.3组大鼠心肌组织中RASSF1A mRNA和蛋白的表达差异均有统计学意义(F=38.968,284.445,P<0.001).与对照组相比,模型组、干预组RASSF1A mRNA和蛋白的表达降低(P<0.05);与模型组相比,干预组RASSF1A mRNA和蛋白的表达升高(P<0.05).结论:替米沙坦可通过调节RASSF1A的表达抑制心肌肥厚的发生.