白藜芦醇对人肾细胞癌786-0细胞周期、凋亡及PDCD5mRNA表达的影响

摘要

Aim:To investigate the effects of reservatrol( RES ) on the cell cycle, apoptosis and expression of PDCD5 mRNA in renal cell carcinoma 786-0 cells. Methods:786-0 cells in the experimental group were cultured with the medium containing 25 μmol/L RES for 24, 48 and 72 h, and the cells in the blank control group were cultured with the medium without RES. The cell cycle phase distribution and the cell apoptosis of 786-0 cells were analyzed by using flow cytometry;and the expression of PDCD5 mRNA was analyzed by using in situ hybridization. Results: 786-0 cells in the experimental group at G1 phase and G2 phase ratio decreased significantly, and the proportion of S phase significantly increased after RES treatment for 24 h. The mRNA level of PDCD5 expression and the number of apoptotic cells in the experimental group were higher than the blank control group at 24,48 and 72 h. Conclusion: RES could upregulate the expression of PDCD5 mRNA in 786-0 cells, which induces cell apoptosis by arresting cells at S phase.%目的:探讨白藜芦醇(RES)对人肾细胞癌786-0细胞周期、凋亡及PDCD5 mRNA表达的影响.方法:取对数生长期的786-0细胞,用含25 μmol/L RES的培养基(实验组)培养24、48及72 h后,应用流式细胞术检测786-0细胞周期和凋亡情况,采用原位杂交技术检测细胞中PDCD5 mRNA的表达情况,均以不含RES的培养基作为空白对照.结果:25 μmol/L RES作用24 h后,实验组786-0细胞G1期及G2期比例显著下降,S期比例明显升高.经25 μmol/L RES处理24、48及72 h后,实验组786-0细胞凋亡细胞数(F组间=869.853,P<0.001)和PDCD5 mRNA的表达均高于空白对照组(F时间=10.067,P<0.001;F组间=649.629,P<0.001),差异有统计学意义.结论:RES可上调人肾细胞癌786-0细胞PDCD5 mRNA的表达,使786-0细胞阻滞在S期而诱导细胞凋亡.