目的：探讨脂肪来源干细胞复合不同生物支架在裸鼠皮下的促血管化作用。方法：根据所选支架的不同，将实验分为4组，A组为脂肪颗粒，B组为透明质酸钠，C组为Ⅰ型胶原支架，分别按体积比1：1与25×106 mL-1的脂肪来源干细胞混合，D组为单纯脂肪来源干细胞，将4组试剂注射到12只裸鼠皮下构建软组织，每只裸鼠各注射4个点，3个月后获取相应标本，测定湿质量、存活率、微血管密度。结果：4组间湿质量、存活率、微血管密度比较，差异均有统计学意义（F＝47．523、48．425和47．619，P均＜0．05），且A、B、C组湿质量、存活率、微血管密度均高于D组（P均＜0．05）。结论：脂肪来源干细胞复合生物支架可加速软组织血管化，且效果优于单纯脂肪来源干细胞。%Aim:To discuss the affection of promoting neovascularization of adipose tissue derived stem cells (ADSCs) compounding with different cellular scaffold in nude mice subcutaneous stratum .Methods:The experiment was divided into four groups depending on the selected scaffold .Group A,B and C were given fat particles, sodium hyaluronate, typeⅠcol-lagen,respectively, mixed with ADSCs at 25 ×106 mL-1 .Group D was given pure ADSCs .Four reagents were injected ran-domly into subcutaneous stratum of 12 nude mice to build soft tissue .Each nude muse was injected 4 points.The appropri-ate specimens were obtained three months later and wet weight , survival rate and microvessel density were detected .Re-sults:The differences were statistically significant regarding the wet weight , survival rate , microvessel density among the four groups(F=47.523,48.425,47.619,P<0.05).The wet weight, survival rate, and microvessel density of group A ,B and C were higher than those of group D (P<0.05).Conclusion: ADSCs compounding with cellular scaffold could pro-mote neovascularization in subcutaneous stratum ,and is better than pure ADSCs .
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