宫颈癌患者外周血中髓源抑制细胞比例变化及CD73的表达

摘要

To analyze the percentages of granulocytic myeloid derived suppressor cells (G-MDSCs),monocytic myeloid derived suppressor cells ( M-MDSCs) and the expression of CD73 on the two subsets cells in peripheral blood of cervical cancer patients .Methods:37 patients with cervical cancer and 21 healthy controls were enrolled in this study .The pattern of CD14 +CD11b-as G-MDSCs, CD11b+CD14 +was made M-MDSCs.Flow cytometry was used to detect the per-centage of the two subsets of MDSCs and the expression of CD 73 on the two subsets in peripheral blood of cervical cancer patients.Results:The percentages of G-MDSCs and M-MDSCs in peripheral blood of cervical cancer patients were higher [(52.5 ±2.9)%,(27.7 ±15.2)%] than those in healthy controls[(20.1 ±9.2)%,(22.0 ±6.3)% ](t=7.584, 2.021,P<0.05);Compared with healthy controls , the expression of CD73 on M-MDSCs in cervical cancer patients was higher[(21.4 ±17.1)%]than that of healthy controls[(5.9 ±5.0)%](t=5.128,P<0.001).The percentages of G-MDSCs and the expression of CD 73 on G-MDSCs didn't correlate to pathologic types , differentiation , clinical stages and lymph node metastasis(P>0.05);The percentages of M-MDSCs in peripheral blood of cervical cancer patients had the re-lationship with clinical stages(F=9.566,P=0.001).The expression of CD73 on M-MDSCs in cervical cancer patients had the relationship with lymph node metastasis and clinical stages (t/F=7.664,2.419,P<0.05).Conclusion:Higher per-centages of MDSCs may contribute to the occurrence of cervical cancer , the higher expression of M-MDSCs may be related to the disease progression .CD73 may be an important molecules for the immunosuppression of MDSCs .%目的：探讨宫颈癌患者外周血中粒细胞样髓源抑制细胞（ G-MDSCs ）和单核细胞样髓源抑制细胞（ M-MDSCs）的比例及其CD73的表达在宫颈癌发病中的作用。方法：收集37例宫颈癌患者和21例健康人外周血，以CD11b＋CD14-为G-MDSCs标志物，CD11b＋CD14＋为M-MDSCs标志物，采用流式细胞术检测两群MDSCs的比例及其CD73表达。结果：宫颈癌组外周血G-MDSCs、M-MDSCs的表达水平[（52．5±2．9）％、（27．7±15．2）％]均高于健康组[（20．1±9．2）％、（22．0±6．3）％]（ t＝7．584、2．021，P＜0．05）；宫颈癌组外周血M-MDSCs上CD73的表达水平[（21．4±17．1）％]高于健康组[（5．9±5．0）％]（t＝5．128，P＜0．001）；宫颈癌组外周血G-MDSCs比例及其CD73的表达与病理类型、临床分期、分化程度、淋巴结转移均无关（P均＞0．05），M-MDSCs比例与临床分期有关（F＝9．566，P＝0．001），其CD73的表达与临床分期和淋巴结转移有关（t/F＝7．664、2．419，P＜0．05）。结论：宫颈癌患者外周血MDSCs的比例与肿瘤发生相关，其中M-MDSCs的水平可能与病情进展有关。