N-乙酰-D-乳糖胺、抗 CD28单克隆抗体对肺癌患者来源的 CIK细胞增殖及杀伤功能的影响

摘要

Aim:To study the influence of LacNAc with or without anti-CD28 mAb on the proliferation and function of CIK cells from patients with lung cancer .Methods:The peripheral blood was collected from 32 patients with lung cancer . The monocytes were extracted and divided into four groups after suspension , control group,anti-CD28 mAb group,LacNAc group,and anti-CD28 mAb combined with LacNAc group(combined group),then cultured as CIK technological process and treated accordingly.The ability of proliferation was measured on the day of 4th, 8th, 10th, 12th, and 14th.IFN-γ,TNF-α, IL-2 and IL-10 secreted by CIK cells were measured on the 14th day.Phenotypic analysis via flow cytometry and anti-tumor affection on K562 cells via CCK-8 assay were performed on the 14th day.Results:The proliferation of CIK cells was signif-icantly enhanced by LacNAc or anti-CD28 mAb.The cytokine secretion,ratio of effector cells(CD3+CD8 +cells) and kill-ing efficiency of CIK cells all improved after being treated by LacNAc or anti-CD28 mAb(P<0.05),but there was no in-teraction between LacNAc and anti-CD28 mAb(P>0.05).Conclusion:It is indicated that CIK cells treated with LacNAc or anti-CD28 mAb exhibit a potential in cytotoxic activity against tumor cells , but there is no interaction between them .%目的：观察N-乙酰-D-乳糖胺联合抗CD28单克隆抗体对细胞因子诱导的杀伤细胞（ CIK细胞）增殖及杀伤功能的影响。方法：提取32例肺癌患者的外周血单个核细胞，分为对照组、抗CD28单克隆抗体组、N-乙酰-D-乳糖胺组及抗CD28单克隆抗体和N-乙酰-D-乳糖胺联合组（联合组），依据CIK细胞培养流程培养和分组处理后于第4、8、10、12、14天测定各组细胞增殖，第14天测定IL-2、IL-10、γ干扰素及肿瘤坏死因子-α的分泌及对K562细胞的杀伤作用。结果：抗CD28单克隆抗体、N-乙酰-D-乳糖胺单独作用均能促进CIK细胞增殖及IL-2及肿瘤坏死因子-α的分泌，增加效应细胞（CD3＋CD8＋细胞）的比例，增强CIK细胞对K562细胞的杀伤能力（P＜0．05），但二者联合没有协同效应（ P＞0．05）。结论：N-乙酰-D-乳糖胺与抗CD28单克隆抗体均能增强CIK细胞增殖及杀伤功能，但二者无协同作用。