慢性阻塞性肺疾病急性加重期和稳定期患者血清IL-8、TNF-α及免疫因子的检测

摘要

Aim:To investigate the changes of serum interleukin-8(IL-8) and tumor necrosis factor α(TNF-α) in pa-tients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods: A total of 72 patients with AECOPD in the Second Affiliated Hospital of Zhengzhou University from February 2015 to December 2016 were selected as AECOPD group,66 cases at COPD stable phase were selected as COPD stable phase group,and 70 healthy volunteers were selected as control group. The serum levels of IL-8,IL-6,TNF-α,C reactive protein(CRP),serum amyloid A(SAA) and interferon G inducible protein 10(IP-10) of each group were determined, and percentage of forced expiratory volume in 1 second(FEV1) and FEV1/forced vital capacity(FVC) were detected.Results: In AECOPD group, IL-8, IL-6, TNF-α, CRP,SAA,and IP-10 were(0.53 ± 0.11) μg/L,(25.39 ± 3.21) μg/L,(8.39 ± 1.48) mg/L,(10.27 ± 1.88) mg/L,(15.12 ± 5.43) mg/L and (110.43 ± 42.41) ng/L, which were significantly higher than those of the COPD stable phase group and control group(P <0.05). In AECOPD group, FEV1 and FEV1/FVC were (46.93 ± 4.22)% and (38.90 ± 4.01)%, which were significantly lower than those in the COPD stable phase group and control group(P <0.05). The CAT of patients with AECOPD was(18.41 ± 2.55). Serum IL-8,IL-6,TNF-α,CRP,SAA and IP-10 were negatively correlated with FEV1 and FEV1/FVC(r= -0.551- -0.454,P<0.05). CAT score was positively correlated with IL-8,IL-6,TNF-α,and CRP(r=0.481-0.601,P<0.05).Conclusion:In AECOPD patients,serum IL-8,IL-6, TNF-α,CRP,SAA,and IP-10 increase,which suggests poor lung function and severity of the disease.%目的:探讨慢性阻塞性肺疾病急性加重期(AECOPD)患者血清白细胞介素-8(IL-8)、肿瘤坏死因子α (TNF-α)等因子水平的变化.方法:选取2015年2月至2016年12月郑州大学第二附属医院收治的AECOPD患者72例(AECOPD组)、COPD稳定期患者66例(COPD稳定期组),同时选取健康志愿者70例作为对照组,检测各组血清IL-8、IL-6、TNF-α、C反应蛋白(CRP)、淀粉样蛋白A(SAA)和干扰素G诱导蛋白10(IP-10),同时检测第1秒用力呼气容积占预计值百分比(FEV1)和FEV1/用力肺活量(FVC).结果:AECOPD组IL-8、IL-6、TNF-α、CRP、SAA和IP-10分别为(0.53 ± 0.11) μg/L、(25.39 ± 3.21) μg/L、(8.39 ± 1.48) mg/L、(10.27 ± 1.88)mg/L、(15.12 ± 5.43) mg/L和(110.43 ± 42.41) ng/L,高于COPD稳定期组和对照组(P均<0.05).AECOPD组FEV1和FEV1/FVC分别为(46.93 ± 4.22)%和(38.90 ± 4.01)%,低于COPD稳定期组和对照组(P均<0.05).AECOPD患者CAT为(18.41 ± 2.55)分;IL-8、IL-6、TNF-α、CRP、SAA 和 IP-10与 FEV1、FEV1/FVC 呈负相关(r = -0.551 ~-0.454,P均<0.05),CAT评分与IL-8、IL-6、TNF-α和CRP呈正相关(r=0.481~0.601,P均<0.05).结论:AE-COPD患者血清IL-8、IL-6、TNF-α、CRP、SAA和IP-10水平升高,可导致患者肺功能下降、疾病加重.