Testing of Kidney Transplant Patients with Leukocyte Typing Sera and Production of Grouped Antisera

摘要

Data on the specificities of 16 grouped antisera are given. A total of 240 retrospective kidney transplant pairs were tested by a lymphotoxic test. Patients who were matched for the major leucocyte groups survived at a higher rate than patients who were mismatched with their donors. Prospective kidney transplant recipients and donors were tested before transplantations. A program by which the 2 best matched recipients are selected out of a pool of patients for each available cadaver donor was initiated. The recipients were all pretyped and maintained by hemodialysis. Out of 43 prospective recipients and 10 prospective donors, 3 kidneys were transplanted. Leucocyte typing sera received from the NIH Serum Bank are being tested with kidney transplant patients and their donors. The lymphocyte cytotoxicity tests were utilized to study horse anti-human lymphocyte sera. The ALS sera have a markedly variable titer on different recipients, and currently this titer is being compared to the biologic effectiveness of the sera in kidney transplant recipients. A rapid metabolic screening test was developed to assess the viability of cadaver kidneys. A method was developed which provides pure lymphocyte suspensions with the minimum damage. An automatic locating device for recording the location of 60 droplets on a single microchamber was developed. A disposable plastic chamber for performance of the microdroplet test was designed and the tooling is now being made for commercial production. Improved methods of statistical analysis in typing were also devised. Red cell antigens were studied in 100 transplant patients. Cross checking of typing results with other laboratories was performed. (Author)