Oasis MCX固相萃取柱结合内标法高效检测羊肉瘦肉精残留量的方法研究

摘要

The study aimed to establish the detection method of clenbuterol in lamb by using Oasis MCX SPE column combined internal standard method.The separation was performed by using acetonitrile-0.1% formic acid solution as mobile phase and 5% ammoniated methanol as eluent.The detection was conducted by using HPLC-ESI-MS/MS in positive ion and multiple reaction monitoring (MRM) modes.The results showed that this method possessed satisfactory detecting rate for clenbuterol ranging from 0.050 ng/mL to 5.000 ng/mL.The coefficient was 0.997 8, the recovery rate was 98%~102%, and the relative standard deviation was 2.09%.With high sensitivity and accuracy, this method could be used for detecting the clenbuterol in lamb, and it also had certain guiding significance for quantitative analysis of clenbuterol in other meat products.%在以乙腈-0.1%甲酸溶液为流动相、5%氨化甲醇为洗脱剂、梯度洗脱、HPLC-MS/MS电喷雾正离子、多反应监测模式的检测条件下,利用高效液相色谱串联三重四级杆质谱仪,研究了Oasis MCX固相萃取柱结合内标法检测羊肉中克伦特罗(俗称瘦肉精)残留量的方法.结果表明,克伦特罗残留量在线性范围 0.050~5.000 ng/mL 内均具有良好的检出率,相关系数为0.9978,加标回收率为98%~102%,相对标准偏差为2.09%.该方法灵敏度高,检测结果准确度高,适用于羊肉中克伦特罗残留的定量检测,对其他肉质中克伦特罗的检测也具有一定的参考价值.