粗柄羊肚菌转录组的SSR分布和序列特征分析

摘要

The transcriptome of Morchella crassipes M10 collected from Xinzheng of He'nan Province was sequenced by the next generation sequencing technology,and distribution characteristic of simple sequence repeats (SSR) were analyzed.The results showed that: 1)A total of 13 044 SSR sites were detected,which was distributed in 8851 Trinity genes,about 2858 Trinity genes had more than one SSR loci and the number of compound formation SSR loci was 1925.The average density of SSR loci was 2067.77 bp/piece in the transcriptome sequence of Morchella crassipes,which was much higher than that of other fungi. 2)The number of single base repeat motif was 8600, the largest one, among which the number of A/T repeat types was higher than C/G repeat types and the same as most macro-fungi；the number of tri-nucleotide (TGC) repeat motif was 2497, among which ACC/GGT，AGC/CTG and AGG/CCT accounted for 60% and obviously appeared with GC high probability. In the development of SSR molecular marker or other repeat polymorphic marker, SSR abundant with GC could be chosen as the best primers.%基于第二代转录组测序技术,对采集自河南省新郑市的粗柄羊肚菌(Morchellacrassipes)M10菌株进行了深度转录组测序,分析了全转录组简单重复序列SSR(simplesequencerepeat)分布和序列特征.结果表明:1)总计检测到13044个SSR位点,分布在8851条Trinitygenes中,SSR在转录组序列中的平均密度为2067.77bp/个,远高于其他真菌,其中2858条Trinitygenes中包含超过1个SSR位点,复合型SSR位点1925个.2)单碱基重复基元最多,为8600个,其中A/T重复类型远高于C/G重复类型,与多数大型真菌相同;其次是三碱基重复基元,为2497个,其中ACC/GGT,AGC/CTG和AGG/CCT重复类型占60%以上,且明显以GC的高频概率出现,在SSR分子标记或其他重复序列多态性标记的开发上,可优选富含GC的SSR设计引物.