氟虫腈是新一代苯基吡唑类杀虫剂,近年来关于氟虫腈的生态安全和健康风险的研究已成为热点,但有关氟虫腈与环境中其他污染物的复合污染的报道甚少.实验以氟虫腈和铜为研究对象,以斑马鱼胚胎为实验模型,采用双因子方差分析法研究氟虫腈和铜对斑马鱼早期发育的联合毒性.单一毒性结果表明:氟虫腈能够引起胚胎、仔鱼的死亡,以及仔鱼脊柱弯曲.而铜离子对胚胎死亡及畸形没有显著影响,但能够推迟胚胎的孵化.联合毒性结果显示:氟虫腈和铜离子对胚胎孵化的联合作用显著(p＜0.05),两者表现为协同作用；对胚胎死亡和脊柱弯曲的联合作用不明显(p＞0.05),两者表现为相加作用.研究结果为准确评价氟虫腈在实际环境中的复合污染提供依据.%Fipronil, a widespread used phenylpyrazole insecticide, is highly concerned on the eco-safety and human healthy. However, few reports have been issued on the joint effects between fipronil and other contaminants. In this study, the joint effect of fipronil and Cu2+ on the development in zebrafish embryo and larvae was studied by two-factor analysis of variance methods. The results showed that embryos exposed to fipronil displayed mortality and curved body axis, whereas only hatching process inhibition was observed when exposed to Cu2+. Moreover, significant interaction on inhibiting the hatching of zebrafish embryo was observed when exposed to the mixture of fipronil and Cu2+ suggesting synergistic effect between them. No significant interaction existed on the mortality and curved body axis indicating additive effect between them. The results provide substantial knowledge towards identifying and evaluating the environmental risk of fipronil.
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