Objective:To investigate the protective effects of carnosine against experimental closed head injury (CHI) in mice.Methods:The CHI model was established by free-falling weight-drop.Carnosine (250 mg/kg or 500 mg/kg) was administered intraperitoneally 30 min before brain trauma,then q.d for 7 d; while normal saline was administrated for control group.The neurological defect was evaluated by neurological severity score (NSS) within 7 d; the survival rate and the histological alternations were observed.Results:Carnosine prevented the body weight loss of mice at dose of 500 mg/kg; significantly increased the survival rate,and reduced the neurological defect and histological damage at dose of 250 and 500 mg/kg.Conclusion:Carnosine can attenuate closed head injury in mice.%目的:探索肌肽对小鼠闭合性脑损伤的保护作用.方法:建立自由落体法致小鼠闭合性脑损伤模型.从术前30 min开始,二肌肽组经腹腔分别注射肌肽250 mg/kg和500 mg/kg剂量,溶剂对照组及假手术组腹腔注射等量生理盐水.小鼠闭合性损伤后每日1次给药,连续实验7d,并每天进行神经受损程度评分(NSS),记录小鼠生存率及体重改变.实验结束后取小鼠脑组织,切片、HE染色.结果:溶剂对照组、250 mg/kg肌肽组和500 mg/kg肌肽组小鼠闭合性脑损伤7d后的最终存活率分别为50.0％、68.2％和82.4％；与溶剂对照组比较,250 mg/kg肌肽可显著降低小鼠闭合性脑损伤术后第5～7天的NSS(P ＜0.05),500 mg/kg肌肽可显著降低术后第1天及第4～7天的NSS(P＜0.05)；小鼠脑组织切片结果提示肌肽可显著减轻脑组织的组织病理学损伤.结论:肌肽对小鼠闭合性脑外伤具有保护作用,能提高损伤后小鼠生存率并促进神经功能的恢复.
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