姜黄素对自发性高血压大鼠缺血再灌注后视网膜细胞凋亡及p53表达的影响

摘要

[Objective]To investigate the apoptosis and the expression of p53 in retina of spontaneously hypertensive rat(SHR) after retina ischemia reperfusion(RIR) and the effect of curcumin on apoptosis and the expression of p53 in retina. [Methods] Isch-emic reperfusion injury model was used. Male SHR were randomly divided into sham group(SH), ischemia reperfusion group(IR),curcumin group(CU) and solvent control group(SC). Each group was subdivided into 2h, 6h, 24h, 72h and 7d after reperfusion respective-ly(n=9). Retina and the retinal capillary apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end label ing(TUNEL) methods and the caspase-3 activity was evaluated by caspase-3 spectrophotometric analysis. The expression of p53 was determined by streptavidin-perosidase(SP) immunohistochemistry. [Results] The number of apoptosis in retina and the retinal capillary was smaller in CU group than in IR group obviously. The expression of p53 was less in retina in CU group than in IR group. [Conclusions] Curcumin can protect retina and retinal capillary of SHR against RIR. It may be one of the protective mechanisms by modulating the expression of apoptosis related gene p53.%[目的]探讨姜黄素对自发性高血压大鼠缺血再灌注后视网膜细胞凋亡及凋亡相关基因p53表达的影响和意义.[方法]制作大鼠视网膜缺血再灌注(retinal ischemia reperfusion,RIR)模型,将自发性高血压大鼠(spontaneously hypertensive rat,SHR)随机分为假手术组(SH)、缺血再灌注组(IR)、姜黄素处理组(CU)和时照组(SC),每组又分为再灌注后2h、6h、24h、72h和7d等5个亚组(n=9).用TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling)法观察大鼠视网膜和视网膜毛细血管细胞凋亡,分光光度法检测视网膜Caspase-3酶表达活性,用免疫组织化学SP法检测再灌注后不同时段视网膜组织中p53蛋白表达变化.[结果]与IR组比较,CU组能显著减少视网膜和视网膜毛细血管的细胞凋亡数量(P＜0.01),抑制p53蛋白表达(P＜0.01).[结论]姜黄素对SHR视网膜缺血再灌注损伤有保护作用,调控凋亡相关基因p53蛋白的表达可能是其作用机制之一.