马钱子生物碱组分对类风湿性关节炎滑膜细胞增殖作用的比较研究

摘要

[Objective]To observe the effects of Strychnos alkaloids and its different components on proliferation of Human Fibroblast-Like Synoviocytes-Rheumatoid Arthritis(HFLS-RA). [Methods] Different concentrations of Strychnos alkaloids, brucine and strychnine were added to HFLS-RA in vitro for 24h, and their effects on HFLS -RA proliferation were investigated by BRDU colorimetry. [Results] Different concentrations of Strychnos alkaloids showed inhibitory effects on HFLS-RA. A significantly lower inhibitory rate was observed just at concentration of 0.22mg·mL-1 (P<0.05), higher inhibitory rates at other concentrations in brucine group as compared to strychnine group at the same concentration level- The inhibitory rates of non-strychnine components were investigated indirectly by subtracting the inhibitory rate of strychnine from those of Strychnos alkaloids, which were sigrafi-candy lower than brucine group(P<0.01 ,P<0.01 ) and strychnine group(P<0.01 ,P<0.05) when the level of strychnine and brucine was 0.22mg·mL-1 or 0.l1mg·mL-1, but higher than brucine group (P<0.0l) and lower than brucine group with no statistical differences at other levels. (Conclusion)Strychnos alkaloids, strychnine and brucine have obviously inhibitory effects on HFLS-RA proliferation, and brucine shows a better inhibitory effect than strychnine with the decreasing concentration. The inhibitory effect on HFLS-RA proliferation is not observed in non-brucine and non-strychnine components of Strychnos alkaloids.%[目的]比较观察马钱子总生物碱不同组分对类风湿性关节炎(rheumatoid arthritis,RA)滑膜细胞增殖的影响.[方法]体外培养人类风湿性关节炎成纤维样滑膜细胞(HFLS-RA),加入不同浓度的马钱子总生物碱、马钱子碱和士的宁,采用溴脱氧尿苷(bromodeoxyuridine,BRDU)比色法观察马钱子不同生物碱组分对细胞增殖的影响.[结果]不同浓度的马钱子总生物碱对HFLS-RA细胞增殖表现出良好的抑制作用.同浓度水平组比较,仅在浓度水平为0.22mg·mL-1时,马钱子碱组对细胞增殖的抑制率明显低于士的宁组(P＜0.05),其余各浓度水平时均高于士的宁组.以马钱子总生物碱对细胞增殖的抑制率减去士的宁的抑制率,间接反应非士的宁生物碱对该细胞增殖的抑制率,在士的宁和马钱子碱浓度水平均为0.22mg·mL-1或0.11mg·mL-1时,明显低于士的宁组(P＜0.01,P＜0.05)与马钱子碱组(P＜0.01,P＜0.01),其余浓度水平时,均高于士的宁组的抑制率(P＜0.01),低于马钱子碱纽的抑制率但无显著性差异.[结论]马钱子总生物碱、士的宁、马钱子碱对类风湿性关节炎滑膜细胞的增殖均有良好的抑制作用；随着浓度的降低,马钱子碱对滑膜细胞增殖的抑制作用优于士的宁；在马钱子总生物碱中存在的非马钱子碱、非士的宁的生物碱组分未表现出对HFLS-RA细胞增殖的抑制作用.