采用超声波细胞破碎法和索氏提取法提取马齿苋中的总黄酮,用比色法测定总黄酮含量,通过正交试验确定超声波细胞破碎法的最佳提取条件。结果表明,超声波细胞破碎法提取的影响因素顺序为:料液比〉乙醇浓度〉提取时间;用超声波细胞破碎法提取马齿苋中总黄酮的最佳条件为:料液比为1 g∶20 mL、乙醇体积分数为90%、提取时间40 min。测得马齿苋中总黄酮含量为5.63%。%Through ultrasionic cell-break method and Soxhlet extractions to extract the flavonoids in Portulaca oleracea L.and determinate its contents by UV spectrophotometry,the best condition of the extraction was selected by using orthogonal experiment.The results showed that factors influencing the extraction were in the order as follows:solid-liquid ratioethanol concentrationextractive time;the optimal extracting conditions extracted the flavonoids in Portulaca oleracea L.by the Ultrasionic cell-break method and Soxhlet extractions is:solid-liquid ratio 1:50(g/mL),ethanol concentration 90%and extractive time 40min.The contents of flavonoids from the Portulaca oleracea L.in Yan'an were 5.63%.
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