加巴喷丁对异氟烷镇痛和遗忘作用的影响

摘要

目的 观察加巴喷丁(gabapentin,GBP)对异氟烷(isoflurane,Iso)镇痛和遗忘作用的影响.方法 160只小鼠按分层随机区组设计分为镇痛组(热板法和甩尾法)和学习记忆组(避暗实验和跳台实验)2组,每种实验方法组又分为4个亚组:生理盐水组(NS组,生理盐水灌胃,2 h后腹腔注射生理盐水)、GBP组(G组,GBP 25 mg·kg-1灌胃,2 h后腹腔注射生理盐水)、ISO组(I组,生理盐水灌胃,2 h后腹腔注射异氟烷)、GBP+ISO组(GI组,GBP 25 mg·kg-1灌胃,2 h后腹腔注射异氟烷)组,每组10只.热板法和甩尾法观察各组小鼠痛阈值差异,避暗实验和跳台实验观察各组小鼠潜伏期和错误次数差异.结果 在热板实验和甩尾实验中,与I组相比,GI组热板痛阈(pain threshold in hot-plate test,HPPT)或甩尾潜伏期(tail-flick latency,TFL)延长(P＜0.05或P＜0.01);在避暗实验和跳台实验中,GBP+ISO组较NS组潜伏期缩短、错误次数增加(P＜0.05或P＜0.01),但较I组差异无统计学意义(P＞0.05).结论 加巴喷丁可增强异氟烷的镇痛作用,但25 mg·kg-1加巴喷丁灌胃对异氟烷遗忘作用无明显影响.%Objective To observe the influence of gabapentin ( GBP) on the analgesia and amnesia effects of isoflurane (ISO) on mice. Methods 160 mice were divided by a stratified randomized block design into analgesic experimen - tal group ( hot - plate test and hot water tail - flick test) and learning and memory experimental group ( step - through test and step -down test) and each test group was further divided into 4 subgroups (re = 10 each) : NS group, GBP (G) group, ISO (I) group, GBP + ISO (GI) group. Pain threshold in hot plate (HPPT), tail - flick latency (TFL) in tail flick test, step - through latency , step - down latency and error times in learning and memory experiments were observed . Results In hot - plate test and tail - flick test, compared with I group, the HPPT or TFL of GI group improved (P <0. 05 or P <0. 01) ; in step - through test and step - down test, compared with NS group , the latency of GI group shortened and error times increased (P <0. 05 or f < 0. 01) but was not statistically significant compared with I group ( P > 0. 05 ). Conclusion GBP can enhance the analgesic effects of ISO but 25 mg· kg-1 GBP by intragastric administration has no significant influence upon the amnesia effect of ISO on mice .