SMYD3 siRNA 对人脑胶质瘤细胞迁移、侵袭和增殖的影响

摘要

目的：探讨 SET －及 MYND －结构域含有蛋白3（SMYD3）siRNA 对胶质瘤细胞迁移、侵袭和增殖的影响。方法将 Control siRNA、SMYD3 siRNA 分别转染脑胶质瘤 U87细胞，Western blot 实验检测 SMYD3蛋白表达情况，CCK －8细胞增殖实验检测沉默 SMYD3对 U87细胞增殖的影响，Transwell chamber 细胞迁移、侵袭实验观察沉默 SMYD3对 U87细胞迁移、侵袭的影响。结果SMYD3 siRNA 组 SMYD3蛋白相对表达量为0．377±0．252，Control siRNA 组为0．843±0．02；CCK －8细胞增殖实验中，24、48、72、96 h SMYD3 siRNA 组细胞的光密度（D）值分别为0．677±0．130、0．920±0．061、1．495±0．01、1．986±0．138，Control siRNA 组分别为0．375±0．041、0．649±0．071、0．944±0．08、1．256±0．130；Transwell chamber 细胞迁移、侵袭实验中，SMYD3 siRNA 组细胞迁移能力下降了36．7％，细胞的侵袭能力下降了约41．9％（P 均＜0．05）。结论抑制 SMYD3表达后，胶质瘤 U87细胞的增殖、迁移和侵袭能力下降。%Objective To investigate the effects of SET and MYND domain containing 3 (SMYD3) small interfering RNA (siRNA) on the migration, invasion and proliferation of glioma cells.Methods Both control siRNA and SMYD3 siRNA were transfected into U87 glioma cells.Then, the amount of SMYD3 in U87 glioma cells was detected by Western blotting.The proliferation of U87 glioma cells after SMYD3 knockdown was measured by CCK -8 assay.Furthermore, the effects of SMYD3 on the migration and invasion of U87 glioma cells were determined using Transwell chamber and in-vasion assay.Results The relative amount of SMYD3 was 0.377 ±0.252 in the silencing group and 0.843 ±0.02 in the control.According to CCK -8 assay, after 24 h, 48 h, 72 h and 96 h of cultivation, the D values of SMYD3 siRNA/control siRNA were 0.677 ±0.130/0.375 ±0.041, 0.920 ±0.061/0.649 ±0.071, 1.495 ±0.01 /0.944 ±0.08 and 1.986 ±0.138 /1.256 ±0.130, respectively.After SMYD3 silencing, U87 glioma cells presented remarkably decreases in its abilities to migration (36.7%, P <0.05) and invasion (41.9%, P <0.05).Conclusion Knockdown of SMYD3 can suppress the migration, invasion and proliferation of U87 glioma cells.