蜂蜜对吗啡所致小鼠便秘及镇痛作用的影响

摘要

目的：观察不同浓度蜂蜜对吗啡所致小鼠便秘及吗啡镇痛效果的影响。方法50只小鼠随机分为5组（n ＝10）：空白组、模型组（吗啡＋生理盐水组）、低剂量组（吗啡＋20％蜂蜜组）、中剂量组（吗啡＋40％蜂蜜组）、高剂量组（吗啡＋80％蜂蜜组），每组10只。用吗啡建立便秘模型，以每组小鼠72 h 排便重量，碳粉溶液在小鼠小肠内的推进距离占小肠全长百分数为指标，观察蜂蜜对吗啡所致便秘的影响；采用热板法判断蜂蜜对吗啡镇痛作用的影响。结果与模型组相比，蜂蜜可增加小鼠粪便的重量及小肠的推进率（P ＜0．05）；与模型组相比，蜂蜜可增强吗啡的镇痛作用，但仅高剂量蜂蜜与其的差异有统计学意义（P ＜0．05）。结论蜂蜜可缓解吗啡所致便秘，增强吗啡的镇痛作用。%Objective To investigate the effects of honey at different concentrations on the constipation and analge-sia induced by morphine.Methods A total of 50 Kunminmg mice were randomly divided into five groups (n =10), namely a blank group, a model group (morphine +normal saline), a low -dose group (morphine +20% honey), a mid-dle -dose group (morphine +40% honey) and a high -dose group (morphine +80% honey).A model of constipation was established in mice through administration of morphine.Then, the effects of honey on morphine -induced constipa-tion were assessed, according to 72 -hous defecation weight and the ratio of the moving distance by carbon powder solu-tion to the whole length of the small intestine.The analgesic effects produced by morphine were determined by the beat plate board method.Results After the administration of honey, the mice could produce increased amounts of defecation and stimulate the movement of the intestine (P <0.05), compared with the model group.Honey treatment could enhance pain tolerance in mice exposed to morphine, which however showed significant difference at the high dose (P <0.05). Conclusion Honey can relieve the constipation induced by morphine and enhance pain tolerance.