目的 探讨E26转录因子-1(Ets-1)和信号转导与转录激活因子6(STAT6)在食管病变组织中的表达,并分析其相关性与临床意义.方法 应用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连结法和反转录聚合酶链反应法检测36例食管鳞癌、36例癌旁非典型增生及18例正常食管黏膜组织中Ets-1、STAT6的表达.结果 Ets-1、STAT6蛋白在正常食管黏膜、癌旁非典型增生及食管鳞癌组织中阳性表达率均依次升高,组间比较差异均具有统计学意义(P＜0.05),且二者呈正相关关系(r =0.447,P＜0.05).Ets-1、STAT6 mRNA在正常食管黏膜、癌旁非典型增生及食管鳞癌组织中相对含量依次升高,组间比较差异均具有统计学意义(P＜0.05).Ets-1及STAT6异常表达与食管癌的分级、浸润深度及淋巴结转移均密切相关(P＜0.05).结论 Ets-1和STAT6异常表达与食管上皮癌变有关,二者可能具有协同作用,并可作为判断患者预后的分子指标.%Objective To explore expression of E26 transformation-specific-1 (Ets-1) and signal transducers and activators of transcription 6(STAT6) in esophageal lesions,and to analyze the correlation with clinicopathologic characteristics. Methods The expression levels of Ets-1 and STAT6 protein and mRNA were detected in 36 cases of esophageal squamous cell carcinoma( ESCC) ,36 cases of atypical hyperplasia and 18 cases of normal esophageal tissues by immunohistochemistry streptavidin-peroxidase and reverse transcription-polymerase chain reaction. Results Positive expression rates of Ets-1 and STAT6 proteins were gradually increased from normal esophageal tissues, atypical hyperplasia to ESCC tissues, there were significantly difference among the groups(P <0. 05) ,and their expressions were positively correlated(r =0. 447 ,P <0. 05) . The relative amount of Ets-1 and STAT6 mRNAs were gradually increased from normal esophageal tissues, atypical hyperplasia to ESCC tissues, there were significantly difference among the groups ( P < 0. 05 ). The abnormality expression of Ets-1 and STAT6 were positively correlated with tumor grade, infiltration and lymphatic metastasis of ESCC tissue. Conclusion The abnormal expression of Ets-1 and STAT6 are related to esophageal carcinogenesis, have synergistic effect and can be used as the prognostic molecular markers.
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