慢性铝中毒对大鼠端脑和海马中糖原合成酶激酶3β的影响

摘要

Objective To investigate the expression of glycogen synthase kinase 3 beta( GSK-3β) in rat endbrain and hippocampi with chronic aluminum poisoning. Methods Sixty female SD rats,6 months old, were randomly divided into normal group,aluminum taking orally group and intraperitoneal injection aluminum group,20 rats in each group. Normal group was fed with general forage. Aluminum taking orally group was given forage containing AlCl3 · 6H2O 25 mg · g-1. Intraperitoneal injection aluminum group was injected sterilized 9 g · L-1 AlCl3 · 6H2O solution,3 times a week. Three months later,rats were sacrificed by femoral artery bleeding. The expression of GSK-3 p protein in hippocampus was detected by enzyme-linked immu-nosorbent assay and the expression of GSK-3β gene in endbrain and hippocampi was detected by reverse transcription polymer-ase chain reaction. Results Compared with the normal group, GSK-3β mRNA showed higher expression in the rat endbrain and hippocampi in both aluminum taking orally group and intraperitoneal injection aluminum group( P < 0. 05 ). The expression of GSK-3β mRNA in intraperitoneal injection aluminum group was higher than that in aluminum taking orally group ( P < 0. 05). Compared with the normal group,there was no significantly difference of GSK-3β protein expression in hippocampi in both aluminum taking orally group and intraperitoneal injection aluminum group( P > 0. 05 ) . Conclusion GSK-3β gene expression level in rat's endbrain and hippocampi obviously increase after chronic aluminum poisoning.%目的 探讨慢性铝中毒大鼠端脑和海马中糖原合成酶激酶3β (GSK-3β)的表达.方法 6月龄雌性SD大鼠60只随机分为正常组、口服铝组和腹腔注射铝组,每组20只.正常组饲喂正常饲料,口服铝组饲喂25 mg·g-1的含A1C13·6H2O饲料,腹腔注射铝组腹腔注射9g·L-1的灭菌A1C13·6H2O溶液,每周3次.3个月后,股动脉放血处死大鼠.酶联免疫吸附测定法检测海马细胞中GSK-3β蛋白的表达；反转录聚合酶链反应检测海马和端脑中GSK-3β基因的表达.结果 与正常组比较,GSK-3β mRNA在口服铝和腹腔注射铝组大鼠端脑和海马中均高表达(P＜0.05)；腹腔注射铝组海马中GSK-3β mRNA表达高于口服铝组(P＜0.05).口服铝组和腹腔注射铝组大鼠海马中GSK-3β蛋白表达与正常组比较差异无统计学意义(P＞0.05).结论 慢性铝中毒后,端脑与海马中GSK-3β基因表达水平均明显升高.