目的：认识与异常黒胆质证相关的体内氨基酸代谢变化机制，探讨从体内氨基酸代谢差异认知维医不同证候的可能性。方法选择225例维医不同证候肿瘤患者，其中异常黒胆质证患者102例，异常胆液质证患者50例，异常粘液质证患者57例，异常血液质证患者16例。采用高效液相色谱法（HPLC）对患者血浆进行19种氨基酸的定量检测，应用 SIMCA‐P ＋软件对各组样本的血浆氨基酸含量进行正交偏最小二乘判别分析（Orthogonal partial least‐squares discriminant analysis ，OPLS‐DA），并对异常黒胆质证患者与其他3种证候患者之间的不同氨基酸含量的差异性采用 SPSS16．0软件进行独立样本的 t 检验。结果与其他3种证候患者比较，异常黒胆质证患者血浆天冬酰胺、胱氨酸、酪氨酸、缬氨酸、异亮氨酸、亮氨酸和苯丙氨酸等氨基酸含量高（P ＜0．05）；丝氨酸和牛磺酸2种氨基酸在异常粘液质证与异常血液质证2种证候患者体内的含量比异常黒胆质证患者低（P ＜0．05）；与异常黒胆质证患者比较，天冬氨酸、苏氨酸和赖氨酸3种氨基酸在异常粘液质证患者血浆中的含量较低（P ＜0．05），而谷氨酸和甘氨酸2种氨基酸在异常血液质证患者血浆中的含量较低（P ＜0．05）。结论采用 HPLC 法进行血浆氨基酸分析可以发现潜在的、与证候有关的代谢机制差异，这将从代谢差异性预测维医不同证候及阐述不同证候发生、发展的机制提供可能性。%Objective To examine amino acid metabolism of abnormal Savda ,and to identify the underlying mechanism of abnormal Savda and assess the diagnostic potential of Uyghur medicine .Methods Plasma a‐mino acid content was tested by high‐performance liquid chromatography (HPLC ) from cancer patients with different syndromes (n = 225) .Orthogonal projection to latent structure with discriminant analysis (OPLS‐DA) was used for classification and prediction of different syndromes in Uyghur medicine ,and the spectral profiles were subjected to a t‐test for statistical significance .Results Compared to patients with other three syndromes ,patients with abnormal Savda had higher level of asparagine ,cystine ,tyrosine , valine ,isoleucine ,leucine and phenylalanine (P < 0 .05) .Patients with abnormal Belghem abnormal Kan had lower level of serine and taurine compared to patients with abnormal Savda (P < 0 .05) .Patients with abnormal Belghem had lower level of aspartic acid ,threonine ,and lysine and patients with abnormal Kan had lower level of glutamate and glycine compared to patients with abnormal Savda (P < 0 .05) .Conclu‐sion These findings suggest that The HPLC‐based plasma‐free amino acid profiling has great potential for detecting and understanding and classification of syndromes in traditional Uyghur medicine .
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