基于蛋白质组学分析研究中医肾虚痰瘀型2型糖尿病的生物学基础

摘要

目的：从蛋白质组学分析探讨中医肾虚痰瘀型2型糖尿病血清蛋白质变化及发生机制，解析中医不同分型间及其与正常人群间蛋白表型的构成差异。方法对100例2型糖尿病患者进行中医辨证分型，分为肾虚痰瘀及非肾虚痰瘀组（包括气阴两虚、脾虚痰瘀、阴虚热盛、肝肾亏虚及气虚血瘀等中医证型）。对所有患者及50例健康人血清进行相对和绝对定量同位素标记技术检测。通过用 mascot 进行搜索、定量分析采集图谱。结果2型糖尿病肾虚痰瘀组与非肾虚痰瘀、健康人相比，血清中多种蛋白质谱差异性变化，包括肾虚痰瘀型组与非肾虚痰瘀型组差异蛋白表达共20种，其中7种蛋白上调，13种蛋白下调；肾虚痰瘀组与健康对照组差异蛋白有36种，其中15种蛋白下调，21种蛋白上调，差异有统计学意义（P ＜0．05）。结论肾虚痰瘀型2型糖尿病患者蛋白质组学成分与非肾虚痰瘀、健康人之间存在显著差异。%Objective To explore the changes in serum protein and mechanism of type 2 diabetes of Shenxu-tanyu syndrome in Traditional Chinese Medicine (TCM)by using proteomics.Methods 100 case of type 2 diabetes patients are grouped as shenxu-tanyu syndrome group and non-shenxu-tanyu syndrome group (in-cluding Qi and Yin deficiency,spleen hypofunction and dampness accumulation,deficiency of yin and ex-cessive heat syndrome,Liver-Kidney Yin deficiency and blood stasis due to qi deficiency).According to TCM syndrome classification.The serum samples from all patients and 50 cases of healthy people were de-tected by using iTRAQ (isobaric tag for relative and absolute quantitation).By using the mascot software, the spectra were collected and analyzed quantitatively.Results Compared with the non-shenxu-tanyu pa-tients and healthy people,the proteomics of shenxu-tanyu patients had markedly changed.There were 20 different expressions of protein in shenxu-tanyu syndrome group and non-group,among which 7 protein expressions were up-regulated,while 13 protein expressions were down-regulated.There were 36 different protein expressions between Shenxu-tanyu syndrome of type 2 diabetes and healthy group,among which 15 proteins down,21 proteins up-regulated.The differences are statistically significant (P <0.05).Conclu-sion The proteomics of shenxu-tanyu syndrome type 2 diabetes are significantly different from non-shentu-tanyu group and healthy people.