不同浓度 DMSO 对 Min6胰岛细胞活力和ROS 产生的影响

摘要

目的：观察不同浓度二甲基亚砜（DMSO）对 Min6胰岛细胞活力和细胞活性氧族（ROS）产生的影响。方法无菌洁净环境下培养 Min6胰岛细胞株，细胞良好生长状态时，分为正常对照组（DMSO 含量为0％）及DMSO 各浓度组，用不同浓度 DMSO（0．005％、0．0125％、0．05％、0．125％、0．25％）干预 Min6胰岛细胞48 h，采用细胞增殖和毒性检测试剂盒（Cell Counting Kit-8，CCK8法）检测细胞活力，采用2′，7′-二氯荧光黄双乙酸盐（DCFH-DA）探针法检测 ROS 的产生水平。结果与正常对照组比较（不含 DMSO），0．005％、0．0125％、0．05％、0．125％，0．25％ DMSO 组细胞活力均降低；0．005％、0．0125％、0．05％、0．125％ DMSO 组间细胞活力差别不大，0．25％DMSO组细胞活力进一步下降，同时 ROS 产生明显增加。结论Min6细胞对 DMSO 敏感，DMSO 浓度水平对细胞活力、ROS 水平有一定影响，建议相关实验时注意各组 DMSO 含量本身齐同可比，以免影响结果判断。%Objective To observe the effects of dimethyl sulfoxide on Min6 islet cell viability and Reactive Oxygen Species Production (ROS).Methods Min6 islet cell was cultured under sterile clean environment condition,then interfered by DMSO of different concentration,Cell Counting Kit-8 was adopted to detect the cell viability,DCFH-DA probe was used to detect the Production of ROS.Results Compared with 0%DMSO group ,Min6 cell viability decreased in group containing DMSO 0.005%,0.0125%,0.05%, 0.125%,0.25%,there was little difference of cell viability between groups containing 0.005%,0.0125%, 0.05%,0.125% DMSO.Cell viability decreased further in 0.25% DMSO group,at the the same time ROS production increased.Conclusion Min6 cell sensitive to DMSO,different concentration of DMSO may have different influence on cell viability and intracellular ROS level.it is suggested that to keep the same con-centration of DMSO in different group or wrong conclusion may be get.