PI3K、ERK、IGF-1R 和 ER 蛋白在宫颈癌细胞株中的表达及意义

摘要

Objective To investigate the expression of PhosPhoinositide 3 kinase (PI3K),extracellular reg-ulated protein coyness(ERK),Insulin-like Growth Factor 1 Receptor (IGF-1R)and Estrogen receptor (ER)in the specific cervical carcinoma cell lines,and to clarify the relationship between the expression of four proteins and HPV16 in the development of cervical carcinoma.Methods The Western blotting was used to detect the substances expression level of IGF-1R,PI3K,ERK and ER proteins in C33a,HaCaT and SiHa.Results The exprssion of IGF-1R,PI3K and ERK in SiHa cell were significantly higher than that in C33a,HaCaT (P <0.05),and the exprssion of ER in the SiHa cell was significantly down-regula-ted compared with C33a (P < 0.05).Conclusion The differences of protein expression levels in PI3K, ERK ,IGF-1R and ER suggested that those maybe associate with the infection of HPV16 in the occurrence of cervical cancer.%目的：研究体外特定细胞株（C33a、HaCaT 及 SiHa）中磷酸肌醇3激酶（PI3K）、细胞外调节激酶（ERK）、胰岛素样生长因子-1受体（IGF-1R）和雌激素受体（ER）蛋白的表达量，分析各蛋白的表达与 HPV16感染致癌的关系。方法培养 C33a、HaCaT 及 SiHa 3种细胞，采用蛋白免疫印迹（Western blot）技术检测 IGF-1R、PI3K、ERK 和 ER 蛋白在3种细胞株中的表达水平。结果IGF-1R、PI3K 和 ERK 蛋白在 SiHa 细胞株中的表达高于在 C33a、HaCaT 细胞株中的表达，差异有统计学意义（P ＜0．05），而在 SiHa 细胞株中 ER 的表达低于在 C33a细胞株中的表达，差异有统计学意义（P ＜0．05）。结论PI3K、ERK、IGF-1R 和 ER 蛋白在宫颈癌细胞株中呈现不同程度的表达，可能与 HPV16感染后宫颈癌变存在一定的关系。